胶原酶（Nordmark）—细胞组织解离应用—无动物源和GMP级可选

库存 ：999

英文名 ：Collagenase

保质期 ：2-5年

供应商 ：上海曼博生物医药科技有限公司

保存条件 ：2°C~ 8 °C

规格 ：1g

产品简述
Nordmark提供高质量胶原酶NB产品，特别适用于从各种组织中分离细胞。严格的生产标准带来可靠的批间一致性和优异的性能，旨在获得高产量的活细胞。


产品介绍
Nordmark致力于生产生物制药活性成分和药物产品，在胰酶和胶原酶的生产方面具有长达30年的历史经验。凭借这些专业知识，该公司已成功地将自己定位于国际市场。Nordmark的技术包括提取生物原材料（例如用于获得胰酶）、微生物发酵（例如用于分离干细胞的胶原酶）和从细胞培养物中提取活性成分（例如用于获得抗体）。


产品特点

• 超过30年的高质量胶原酶专业供应，按照制药级标准进行纯化和生产
• 组织细胞解离的整体解决方案：按照不同种属、组织/器官、细胞类型进行分类

1. 不同规格，纯度，活性的胶原酶以及中性水解酶
2. 从科研级到无动物源GMP级别多重选择，相关GMP产品已在FDA备案DMF
3. 满足绝大多数组织/器官的单细胞解离需求，包括提供敏感细胞解离专用的温和解离酶方案

• 提供优化过的胰岛专用细胞解离方案和产品
• 用户使用友好：提供优化过的细胞解离Protocol
• 高性价比：相同酶活性条件下价格与全球主流品牌相比具有明显优势
• 各类应用的大量文献支持

应用领域

各种科研用途的器官，组织，细胞的解离；具有GMP无动物源等级，可进行临床用途的细胞解离。

按种属划分

人

猪

啮齿动物

其他

按照组织划分

脂肪	肝脏	皮肤
骨	肺	甲状腺
软骨	肌肉	肿瘤
结肠	神经	脐带
胃/肠	胰腺	心脏
生殖系统

按照细胞种类划分

腺泡细胞	上皮细胞	肌肉细胞
脂肪细胞	成纤维细胞	神经元
羊膜细胞	肝细胞	卵母细胞
心肌细胞	胰岛	成骨细胞
软骨细胞	白细胞	雪旺细胞
绒毛膜细胞	肺细胞	干细胞
子宫内膜细胞	肥大细胞	肿瘤细胞
内皮细胞	黑色素瘤细胞

使用方法
关于细胞解离的一般注意事项
一般来说，所需的胶原酶NB浓度取决于组织类型和来源以及分离程序。应将建议浓度视为起点浓度，并应目视监测消化过程的进展，以确定最佳条件。

我们建议按活性（PZ单位）而不是重量使用胶原酶NB。Nordmark保证了批次间的高度一致性；但是，可能会出现小的变化。因此，活动中的剂量可确保细胞分离过程的一致结果。有关优化组织消化的更多信息，请联系我们的技术支持！

关于分离细胞用于移植入人体

组织消化酶是组织工程和人体移植的关键原材料。Nordmark Biochemicals提供GMP级胶原酶NB产品，该产品由德国制药公司Nordmark根据当前GMP指南制造。欲了解更多信息，请联系我们！

以上按照种属，组织器官，细胞类别划分细胞解离需求，均可提供对应胶原酶的型号规格和推荐使用浓度，方法，以及有关参考文献。


胶原酶和水解酶活性比较
Nordmark的产品组合包括几种胶原酶NB和中性蛋白酶NB产品，可根据其纯化水平（未纯化、纯化、高纯化）和应用领域（研究、临床）进行区分。
 

品名	胶原酶活性	蛋白水解副作用
Collagenase NB 4 Standard Grade	+	++
Collagenase NB 5 Sterile Grade
Collagenase NB 6 GMP Grade
Collagenase NB 4G Proved Grade	++	+++
Collagenase NB 7D Gentle Grade	++	+
Collagenase NB 8 Broad Range	+++	+
Collagenase NB 1 Premium Grade	++++	-
Collagenase NB 1 GMP Grade
Collagenase AF-1 GMP Grade

不同阶段使用等级


客户体验

Title	Reference	Cell Type
Cathepsin B Activity Initiates Apoptosis via Digestive Protease Activation in Pancreatic Acinar Cells and Experimental Pancreatitis	Sendler, M. et al. J. Biol. Chem. 2016, 291, 14717-14731.	Acinar cells
Signaling pathways involved in LPS induced TNFalpha production in human adipocytes	Hoareau, L. et al. Journal Inflamm.2010, 7.	Adipocytes
Ceiling Culture-Derived Proliferative Adipocytes are A Possible Delivery Vehicle for Enzyme Replacement Therapy in Lecithin: Cholesterol Acyltransferase Deficiency	Kuroda, M. et al. Open Gene Ther. J. 2011, 4, 1-10.	Adipocytes
Autologous fat transfer with in-situ mediation AIM a novel and compliant method of adult mesenchymal stem cell therapy	Wu, A. Y. and Morrow, D. J. Translat. Med. 2013, 11.	Adipose stem cells (ASC)
Development of fully defined xeno-free culture system for the preparation and propagation of cell therapy-compliant human adipose stem cells	Patrikoski, M. et al. Stem Cell Res Ther. 2013, 4, 27.	Adipose stem cells (ASC)
Xenofree Enzymatic Products for the Isolation of Human Adipose-Derived Stromal/Stem Cells	Carvalho,P. et al. Tiss Eng Part C Methods 2013, 19.	Adipose stem cells (ASC)
Human Adipose-Derived Mesenchymal Stem Cells in Cell Therapy: Safety and Feasibility in Different "Hospital Exemption" Clinical Applications	Veriter, S. et al. Plos One 2015, 10, e0139566.	Adipose stem cells (ASC)
A Standardized Method of Isolating Adipose-Derived Stem Cells for Clinical Applications	Raposio, E. et al. Annals Plast. Surg. 2015, 00.	Adipose stem cells (ASC)
Use of Bioengineered Bone for Sinus Augmentation	Beaumont, C. et al. J Periodontol 2008, 79, 541-548.	Bone
A nonaggressive, highly efficient, enzymatic method for dissociation of human braintumors and braintissues to viable singlecells	Volovitz, I. et al. BMC Neurosci. 2016, 17, 1-10.	Brain Cells
Cerebral endothelial cells release TNF-alpha after stimulation with cell walls of Streptococcus pneumoniae and regulate inducible nitric oxide synthase and ICAM-1 expression via autocrine loops	Freyer, D. et al. J. Immunol. 1999, 163, 4308-4314.	Cerebral endothelial cells
Atoh8 acts as a regulator of chondrocyte proliferation and differentiation in endochondral bones	Schroeder, N. et al. Plos One 2019, 14, e0218230.	Chondrozytes
Severe hepatic injury in interleukin 18 (IL-18) transgenic mice: a key role for IL-18 in regulating hepatocyte apoptosis in vivo	Finotto,S. et al. Gut 2004, 53, 392-400.	Hepatocytes
Hepatocyte differentiation of mesenchymal stem cells from human adipose tissue in vitro promotes hepatic integration in vivo	Aurich, H. et al. Gut 2009, 58, 570-581.	Hepatocytes
Cold Storage of Rat Hepatocyte Suspensions for One Week in a Customized Cold Storage Solution – Preservation of Cell Attachment and Metabolism	Pless-Petig, G. et al. PlosOne 2012, 7, e40444.	Hepatocytes
Comparison of methods for isolating primary hepatocytes from mini pigs	Li, Y. et al. Xenotransplantation2016, 0, 1-7.	Hepatocytes
Good manufacturing practice and clinical-grade human embryonic stem cell lines	Unger, C.Human Mol. Gen. 2008, 17, R48-R53.	Human embryonic stem cells (hESC)
Teratoma formation by human embryonic stem cells: Evaluation of essential parameters for future safety studies	Hentze, H. et al. Stem Cell Res. 2009, 2, 198-210.	Human embryonic stem cells (hESC)
Intrahepatic Administration of Human Liver Stem Cells in Infants with Inherited Neonatal-Onset Hyperammonemia: A Phase I Study	Spada, M. et al. Stem Cell Rev Rep. 2020, 16, 186-197.	Human Liver Stem Cells (HLSC)
Cryopreservation of human vascular umbilical cord cells under good manufacturing practice conditions for future cell banks	Polchow, B. et al. J Transl Med. 2012, 10, 98.	Human umbilical vein endothelial cells (HUVEC)
Successful Single Donor Islet Allotransplantation in the Streptozotocin Diabetes Rat Model	Jahr, H. Cell Transplant. 2002, 11, 513-518.	Islets of Langerhans
Tyrosine hydroxylase activity in the endocrine pancreas: changes induced by short-term dietary manipulation	Borelli, M. I. et al. BMC Endocr Disord. 2003, 3.	Islets of Langerhans
Influence of Collagenase Loading on Long-Term Preservation of Pig Pancreas by the Two-Layer Method for Subsequent Islet Isolation	Brandhorst, D. et al. Transplant. 2005, 79,38-43.	Islets of Langerhans
Pancreas storage in oxygenated perfluorodecalin does not restore post-transplant function of isolated pig islets pre-damaged by warm ischemia.	Brandhorst, D. et al. Xenotransplant. 2006, 13, 465-470.	Islets of Langerhans
Current status of pancreatic islet transplantation	Merani, S. and Shaprio, A. M. J. Clin Sci (Lond.) 2006, 110, 611-625.	Islets of Langerhans
Quantification of Basal and Stimulated ROS Levels as Predictors of Islet Potency and Function	Armann, B et al. Am J Transplant. 2007, 7,38-47.	Islets of Langerhans
Factors Influencing the Collagenase Digestion Phase of Human Islet Isolation	Kin, T. et al. Transplant.2007, 83, 7-12.	Islets of Langerhans
Improvement of pancreatic islet cell isolation for transplantation	Matsumoto,S. et al. Proc (Bayl Univ Med Cent.) 2007, 20, 357-562.	Islets of Langerhans
Collagenase penetrates human pancreatic islets following standard intraductal administration	Cross, S.E. Transplant. 2008, 86, 907-911.	Islets of Langerhans
Iodixanol-Controlled Density Gradient During Islet Purification Improves Recovery Rate in Human Islet Isolation	Noguchi, H. et al. Transplant. 2009, 87, 1629-1635.	Islets of Langerhans
Pig Pancreas Oxygenation at 20°C Increases Islet ATP Generation but Deteriorates Islet Function	Iken, m. et al. Cell Transplant. 2009, 18, 745-751.	Islets of Langerhans
The Importance of Tryptic-like Activity in Purified Enzyme Blends for Efficient Islet Isolation	Brandhorst, H. et al. Transplant. 2009, 87, 370-375.	Islets of Langerhans
Human Islet Isolation for Autologous Transplantation: Comparison of Yield and Function Using SERVA/Nordmark Versus Roche Enzymes	Anazawa,T. et al. Am J Transplant. 2009, 9, 2383-2391.	Islets of Langerhans
Human Pancreatic Islet Isolation: Part I: Digestion and Collection of Pancreatic Tissue	Qi, M. J Vis Exp. 2009, 26, 1125-1126.	Islets of Langerhans
Low-Temperature Preservation of Isolated Islets is Superior to Conventional Islet Culture Before Islet Transplantation	Noguchi, H. et al. Transplant. 2010, 89, 47-54.	Islets of Langerhans
Experience of islet isolation without neutral protease supplementation	Kin, T. et al. Islets, 2010, 2, 278-282.	Islets of Langerhans
Islet Isolation for Clinical Transplantation	Kin, T. Adv Exp Med Biol. 2010, 654, 683-710.	Islets of Langerhans
A Novel Device for Islte Transplantation Providing Immune Protection and Oxygen Supply	Ludwig, B. et al.Horm Metab Res. 2010, 42, 918-922.	Islets of Langerhans
Agonist of growth hormone-releasing hormone as a potential effector for survival and proliferation of pancreatic islets	Ludwig, B. et al. PNAS 2010, 107, 12623-12628.	Islets of Langerhans
Assessment of human islet isolation with four different collagenases	Shimoda,M. et al. Transplant Proc. 2010, 42, 2049-2051.	Islets of Langerhans
Seven consecutive successful clinical islet isolations with pancreatic ductal injection	Matsumoto, S. et al. Cell Transplant. 2010, 19, 291-297.	Islets of Langerhans
Large-scale Comparison of Libearse HI and Collagenase NB 1 utilized for Himan Islet Isolation	Brandhorst,H. et al. Cell Transplant. 2010, 19, 3-8.	Islets of Langerhans
A Simplified Approach to Human Islet Quality Assessment	Hanson, M. S. et al. Transplant. 2010, 89, 1178-1188.	Islets of Langerhans
Evolution of β-Cell Replacement Therapy in DiabetesMellitus: Islet Cell Transplantation	Jahansouz, C. et al. Diabetes Technol Ther. 2011, 13, 395-418.	Islets of Langerhans
Recent progress in pancreatic islet transplantation	Kuise, t. and Noguchi, H. World J Transplant. 2011, 1, 13-18.	Islets of Langerhans
Impact of the number of infusions on 2-year results of islet-after-kidney transplantation in the GRAGIL network	Borot,S et al. Transplant. 2011, 92, 1031-1038.	Islets of Langerhans
Improved Pancreatic Islet Isolation Outcome in Autologous Transplantation for Chronic Pancreatitis	Naziruddin, B. et al Cell Transplant 2012, 21, 553-558.	Islets of Langerhans
Comparative impact on islet isolation and transplant outcome of the preservation solutions Institut Georges Lopez-1, University of Wisconsin, and Celsior	Niclauss, N. et al. Transplant. 2012, 93, 703-708.	Islets of Langerhans
Fresh Islets Are More Effective for Islet Transplantation Than Cultured Islets	Noguchi, H. et al. Cell Transplant. 2012, 21, 517-523.	Islets of Langerhans
Evaluation of Osmolality of Density Gradient for Human Islet Purification	Noguchi, H. et al. Cell Transplant. 2012, 21, 493-500.	Islets of Langerhans
Impact of Tissue Volume and Purification on Clinical Autologous Islet Transplantation for the Treatment of Chronic Pancreatitis	Matsumoto,S. et al. Cell Transplant. 2012, 21, 625-632.	Islets of Langerhans
Update on Islet Transplantation	McCall, M. and Shapiro, A. M. J. Cold Spring Harb Perspect Med. 2012, 2, a007823.	Islets of Langerhans
Improvement of collagenase distribution with the ductal preservation for human islet isolation	Shimoda, M. et al. Islets 2012, 4, 130-137.	Islets of Langerhans
Isolation of human islets for autologous islet transplantation in children and adolescents with chronic pancreatitis	Bottino,R et al. J Transplant. 2012, 642787.	Islets of Langerhans
Collagenase Does Not Persist in Human Islets Following Isolation	Cross, S. E. Cell Transplant. 2012, 21, 2531-2535.	Islets of Langerhans
Transplanted Functional Islet Mass: Donor, Islet Preparation, and Recipient Factors Influence Early Graft Function in Islet-After-Kidney Patients	Friberg, A. S. et al. Transplant. 2012, 93, 632-638.	Islets of Langerhans
Robot-assisted pancreatoduodenectomy with preservation of the vascular supply for autologous islet cell isolation and transplantation: a case report	Giulianotti, P. et al J Med case rep. 2012, 6.	Islets of Langerhans
Quality of Isolated Pig Islets Is Improved Using Perfluorohexyloctane for Pancreas Storage in a Split Lobe Model	Brandhorst, H. et al. Cell Transplant. 2013, 22, 1477-1483.	Islets of Langerhans
Clinical islet isolation outcomes with a highly purified neutral protease for pancreas dissociation	O'Gorman, D. et al. Islets, 2013, 5, 111-115.	Islets of Langerhans
Transplantation of Encapsulated Pancreatic Islets as a Treatment for Patients with Type 1 Diabetes Mellitus	Qi, M. Adv Med. 2014, 429710.	Islets of Langerhans
Human islet cell isolation: the initial step in an islet transplanting program in Shiraz, Southern Iran	Azarpira,N. et al. Exp Clin Transplant.2014, 12, 139-142.	Islets of Langerhans
Effects of Donor-, Pancreas-, and Isolation-Related Variables on Human Islet Isolation Outcome: A Systematic Review	Hilling, D. et al. Cell Transplant. 2014, 23, 921-928.	Islets of Langerhans
Islet Product Characteristics and Factors Related to Successful Human Islet Transplantation From the Collaborative Islet Transplant Registry (CITR) 1999–2010	Balamurugan, A.N. et al. Am J Transplant 2014, 14, 2595-2606.	Islets of Langerhans
Autologous Islet Transplantation With Remote Islet Isolation After Pancreas Resection for Chronic Pancreatitis	Tai, D.S. et al. JAMA Surg. 2015, 150, 118-124.	Islets of Langerhans
National Institutes of Health–Sponsored Clinical Islet Transplantation Consortium Phase 3 Trial: Manufacture of a Complex Cellular Product at Eight Processing Facilities	Ricordi, C. et al. Diabetes 2016, 65, 3418-3428.	Islets of Langerhans
Enzyme Development for Human Islet Isolation: Five Decades of Progress or Stagnation?	Brandhorst, D. et al. Rev Diabet Stud, 2016, 14, 22-38.	Islets of Langerhans
Quantifying the Effects of Different Neutral Proteases on Human Islet Integrity	Brandhorst, H. et al. Cell Transplant 2017, 26, 1733-1741.	Islets of Langerhans
Evaluation of collagenase gold plus BP protease in isolating islets from human pancreata	Khiatah, B. Islets 2018, 4, 51-59.	Islets of Langerhans
Low Cost, Enriched Collagenase-Purified Protease Enzyme Mixtures Successfully Used for Human Islet Isolation	Loganathan, G. et al. OBM Transplantation 2019, 3.	Islets of Langerhans
Hypoxia-Preconditioned Wharton’s Jelly-Derived Mesenchymal Stem Cells Mitigate Stress-Induced Apoptosis and Ameliorate Human Islet Survival and Function in Direct Contact Coculture System	Keshtkar, S. et al. StemCell Int. 2020.	Islets of Langerhans
Comparison of islet isolation result and clinical applicability according to GMP-grade collagenase enzyme blend in adult porcine islet isolation and culture	Kwak, K. et al. Xenotransplant. 2021, e12703.	Islets of Langerhans
Hepatocyte differentiation of mesenchymal stem cells from human adipose tissue in vitro promotes hepatic integration in vivo	Aurich, H. et al. Gut 2009, 58, 570-581.	Mesenchymal Stem Cells
Nonexpanded mesenchymal stem cells for regenerative medicine - yield in stromal vascular fraction from adipose-tissue	Faustini, M. et al. Tissue Engin. 2010, 16, 1515-1521.	Mesenchymal Stem Cells
A new standardized clinical-grade protocol for banking human umbilical cord tissue cells	Fazzina, R. et al. Transfusion 2015, 00, 1-10.	Mesenchymal Stem Cells
Efficient animal-serum free 3D cultivation method for adult human neural crest-derived stem cell therapeutics	Greiner, J. et al. Eur. Cells Mat. 2011, 22, 403-419.	Neural crest-derived stem cells
Culture bag systems for clinical applications of adult human neural crest-derived stem cells	Greiner, J. et al. Stem Cell Res Ther. 2014, 5, 34.	Neural crest-derived stem cells
Efficient Schwann cell purification by differential cell detachment using multiplex collagenase treatment	Jin,Y-Q. et al. J Neurosci Methods 2008, 170, 140-148.	Schwann Cells
The use of glandular-derived stem cells to improve vascularization in scaffold-mediated dermal regeneration	Egana, J. T. et al. Biomterials 2009, 30, 5918-5926.	Stem cells
Glandular Stem Cells: A New Source for Myocardial Repair?	Guldner, N. W. et al. Chapter in Reg. Medicine and Tiss. Eng. - Cells and Biomat. 2011	Stem cells
Cellular modulation of polymeric device surfaces - promise of adult stem cells for neuro-prosthetics	Richter, A. et al. Front Neurosci. 2011, 5, 1-10.	Stem cells
Validation of an Automated Procedure to Isolate Human Adipose Tissue–Derived Cells by Using the Sepax  Technology	Güven, S. et al. Tissue Engin. 2012, 18, 575-582.	Stromal Vascular Fraction
Development and Validation of a Fully GMP-Compliant Process for Manufacturing Stromal Vascular Fraction - A Cost-Effective Alternative to Automated Methods	Francois, P.Cells 2020, 9, 2158-2182.	Stromal Vascular Fraction
Pluripotency of adult stem cells derived from human and rat pancreas	Kruse,C. et al. Appl Phys.A 2004	Pancreatic stellate-like cells (PSLCs)
Phenotypic heterogeneity among tumorigenic melanoma cells from patients that is reversible and not hierarchically organized	Quintana, E. et al. Cancer Cell 2010, 5, 510-523.	Tumor cells
A nonaggressive, highly efficient, enzymatic method for dissociation of human braintumors and braintissues to viable singlecells	Volovitz, I. et al. BMC Neurosci. 2016, 17,1-10.	Tumor cells