Recombinant Rhesus Macaque ACE-2 (C-10His)

英文名 ：Recombinant Rhesus Macaque ACE-2 (C-10His)

规格 ：10ug/50ug/500ug/1mg

Recombinant Rhesus Macaque ACE-2 (C-10His) (C07U)

 

产品说明(Description)

Recombinant Rhesus Macaque Angiotensin-Converting Enzyme 2 is produced by our Mammalian expression system and the target gene encoding Gln18-Val739 is expressed with a 10His tag at the C-terminus.

Accession #: ACI04564.1

Known as: Angiotensin-Converting Enzyme 2; ACE-Related Carboxypeptidase; Angiotensin-Converting Enzyme Homolog; ACEH; Metalloprotease MPROT15; ACE2

 

制剂(Formulation)

Supplied as a 0.2 μm filtered solution of 25mM Tris-HCl, 150mM NaCl, 1mM ZnCl2, pH 7.5.

 

质量控制(Quality Control)

Purity:        Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

Bioactivity:
Immobilized Rhesus Macaque ACE-2-His(Cat#C07U)at 5μg/ml (100 μl/well) can bind 2019-nCoV S Protein RBD-SD1-mFc(Cat#DRA38). The ED50 of Recombinant 2019-nCoV S Protein RBD-SD1-mFc(Cat#DRA38) is 16.8 ng/ml .

 

运输(Shipping)

The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.

 

保存(Storage)

Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

 

背景(Background)

Angiotensin-Converting Enzyme 2 (ACE-2) is an integral membrane protein and a zinc metalloprotease of the ACE family, the ACE family includes somatic and germinal ACE. ACE-2 cleaves angiotensins I and II as a carboxypeptidase, ACE-2 converts angiotensin I to angiotensin 1-9, and angiotensin II to angiotensin 1-7. ACE-2 is also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. ACE-2 can be high expressed in testis, kidney and heart, in colon, small intestine and ovary at moderate levels. Captopril and lisinopril as the classical ACE inhibitor don’t inhibit ACE-2 activity. ACE-2 may play an important role in regulating the heart function.

 

电泳(SDS-PAGE)

 

参考文献（Reference)

The effect of whey protein on viral infection and replication of SARS-CoV-2 and pangolin coronavirus in vitro[J]. Huahao Fan. et al. ResearchGate. 2020.

 

FOR RESEARCH USE ONLY