jetOPTIMUS®最佳的DNA转染试剂价格_品牌:Polyplus-丁香通官网

库存：现货

英文名：jetOPTIMUS®

CAS号：/

保质期：/

供应商：纽祺（上海）健康科技有限公司

保存条件：5±3℃

规格：0.1ml

Highly efficient: reach maximal gene expression in hard-to-transfect cells 高效:在难以转染的细胞中达到最大的基因表达
Cost-effective: use minimum reagent volume and DNA quantity 成本效益:使用最小的试剂体积和DNA数量
Biologically relevant: keep an excellent cell viability & morphology 生物学相关性:保持良好的细胞活力和形态
Time-saving: transfect with an optimized ready-to-use protocol 省时:使用优化的即时使用协议进行转染

Overview（概述）

Specifications（规范）

Reagent 试剂名称	jetOPTIMUS^® DNA Transfection Reagent jetOPTIMUS® DNA 转染试剂
Molecule delivered 转染核酸	Plasmid DNA 质粒 DNA
Applications 试剂用途	Transient and stable gene expression from plasmid DNA transfection CRISPR Genome editing using DNA approach 大规模病毒生产质粒 DNA 的瞬时基因表达和稳定表达基于DNA方法的CRISPR 基因编辑
Cell types 细胞类型	Adherent hard-to-transfect mammalian cell: - Primary cells (epithelial, hepatocyte, endothelial, fibroblast, etc…) - Stem cells (embryonic, mesenchymal, induced pluripotent) - Cancer cell lines 常规贴壁哺乳动物细胞；难转染的贴壁的哺乳动物细胞：原代细胞（上皮细胞、肝细胞、内皮细胞、成纤维细胞等）干细胞（胚细胞、间叶细胞、诱导多功能干细胞）肿瘤细胞系
Number of transfections 试剂用量	1.5 ml of jetOPTIMUS^® transfection reagent is sufficient to perform 3 000 transfections in 24-well plates or 750 transfections in 6-well plates following the standard protocol 1.5 mL的jetOPTIMUS®转染试剂足以在24孔板上进行3000次转染，或在6孔板上进行750次转染
Storage 储存条件	5°C ± 3°C, stable for 6 months (0.1 mL) to at at least one year (other packaging sizes) when stored appropriately 5℃± 3℃，适当条件储存可稳定至少一年
Provided with 配套提供	jetOPTIMUS^® Buffer jetOPTIMUS® 缓冲液

Summary（总结）

®Ace your DNA transfection in the cell model you need!

As Transfection Experts, we know that it is essential for biologists to work with physiologically relevant cell models. For gene expression studies, transfection is the preferred technique to introduce a gene of interest in a given cell model, due to its cost-effectiveness and simplicity-of-use in comparison to physical techniques (e.g. micro-injection, electroporation).

Several types of cells (primary cells, stem cells or cancer cell lines) remain difficult to transfect with DNA for different reasons: slow-dividing rates, cell fragility and cellular defense mechanisms. To address the current limits of DNA transfection and complement our existing gene expression portfolio (jetPEI®, jetPRIME®, jetMESSENGER®), Polyplus-transfection® engineered an innovative delivery nanoparticle: jetOPTIMUS®.

jetOPTIMUS® is a powerful transfection reagent that improves cellular uptake and endosomal escape of DNA in adherent cells (even in hard-to-transfect cells) resulting in higher transfection efficiency. In order to work in relevant physiological conditions, transfection with jetOPTIMUS® requires a minimum DNA quantity and reagent volume to keep an excellent cell viability and morphology.
®Ace您的DNA转染在您需要的细胞模型!

作为转染专家，我们知道生物学家与生理相关的细胞模型工作是必不可少的。对于基因表达研究，转染是在给定细胞模型中引入感兴趣基因的择优技术，因为与物理技术(如微注射、电穿孔)相比，转染具有成本效益和使用简单性。
由于不同的原因，几种类型的细胞(原代细胞、干细胞或癌细胞系)仍然难以转染DNA:分裂速度慢、细胞脆弱和细胞防御机制。为了解决目前DNA转染的限制，并补充我们现有的基因表达组合(jetPEI®，jetPRIME®，jetMESSENGER®)，polyplus转染®设计了一种创新的传递纳米颗粒:jetOPTIMUS®。
jetOPTIMUS®是一种强大的转染试剂，可以改善粘附细胞(即使是难以转染的细胞)中DNA的细胞摄取和内体逃逸，从而提高转染效率。为了在相关的生理条件下工作，jetOPTIMUS®转染需要最少的DNA数量和试剂体积，以保持良好的细胞活力和形态。

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