Recombinant Mouse IL-13(110AA,C-6His)

英文名 ：Recombinant Mouse IL-13(110AA,C-6His)

规格 ：10ug/50ug/500ug/1mg

Recombinant Mouse IL-13(110AA,C-6His) (CX56)

 

产品说明(Description)

Recombinant Mouse Interleukin-13 is produced by our Mammalian expression system and the target gene encoding Pro22-Phe131 is expressed with a 6His tag at the C-terminus. 

Accession #: P20109

Known as: Interleukin-13; IL-13; T-Cell Activation Protein P600; Il13; Il-13

 

制剂（Formulation）

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

 

质量控制（Quality Control） 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

Bioactivity: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells.

                       The ED50 for this effect is 2-16 ng/ml.

复溶（Reconstitution）

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存（Storage）

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景（Background）

Mouse interleukin 13 (mIL-13) is a pleiotropic cytokine produced by activated Th2 cells. IL-13 induces B cell proliferation and immunoglobin production. It contains a four helical bundle with two internal disulfide bonds. Mouse IL13 shares 58% sequence identity with human protein and exhibits cross-species activity. IL13 signals via receptor IL13R (type2, IL4R) and activates STAT-6. IL13 initially binds IL-13Rα1 with low affinity and triggers association of IL4Rα, generating a high affinity heterodimeric receptor IL13R and eliciting downstream signals. IL13 also binds IL-13Rα2 with high affinity, which plays a role in a negative feedback system of IL13 signaling. IL13 is an important mediator of allergic inflammation and disease.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY