pAd/BLOCK-iT-DEST RNAi Gateway Vector腺病毒质粒价格

保存条件：-20

保质期：2年

英文名：pAd-BLOCK-iT-DEST

库存：100

供应商：上海烜雅生物科技有限公司

CAS号：/

规格：干粉/液体

基本信息
名称：pAd/BLOCK-iT-DEST RNAi Gateway Vector腺病毒质粒
别名：pAd-BLOCK-iT-DEST

原核抗性:	Amp
克隆菌株:	DB3.1
培养条件:	37度

质粒属性

荧光蛋白：
载体宿主：	哺乳细胞,腺病毒
载体用途：	蛋白表达
基因种属：
基因类型：	ORF
原核抗性：	Amp
真核抗性：

质粒简介
pAd/BLOCK-iT-DEST RNAi Gateway Vector腺病毒质粒

质粒图谱

质粒序列
LOCUS Exported 34864 bp ds-DNA circular SYN 17-JUL-2018
DEFINITION synthetic circular DNA
KEYWORDS pAd BLOCK-iT-DEST RNAi Gateway Vector
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 34864)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..34864
/organism="synthetic DNA construct"
/mol_type="other DNA"
repeat_region 1..103
/note="ITR"
/note="inverted terminal repeat of human adenovirus
serotype 5"
misc_signal 191..341
/note="Ad5 Psi"
/note="packaging signal for adenovirus serotype 5"
protein_bind 512..636
/gene="mutant version of attR"
/bound_moiety="LR Clonase(TM)"
/note="attR1"
/note="recombination site for the Gateway(R) LR reaction"
promoter 661..691
/note="lac UV5 promoter"
/note="E. coli lac promoter with an ""up"" mutation"
CDS 745..1404
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
CDS 1746..2051
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/note="ccdB"
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLL-SDK
VSRELYPVVHIGDESWRMMTTD-MASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
protein_bind complement(2092..2216)
/gene="mutant version of attR"
/bound_moiety="LR Clonase(TM)"
/note="attR2"
/note="recombination site for the Gateway(R) LR reaction"
misc_feature 5766..5869
/note="Ad SA"
/note="adenovirus major late transcript splice acceptor
(Friedrich and Soriano, 1991)"
repeat_region 32680..32782
/note="ITR"
/note="inverted terminal repeat of human adenovirus
serotype 5"
rep_origin complement(33006..33594)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(33746..34606)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPA?MATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(34607..34711)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga
301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg
361 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc
421 cgggtcaaag ttggcgtttt attattatag tcagtcgaag cttggatccg gtacctctag
481 aattctcgag cggccgctag cgacatcgat cacaagtttg tacaaaaaag ctgaacgaga
541 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
601 ataatactgt aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc
661 tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga tccggcgaga
721 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
781 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
841 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
901 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
961 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
1021 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
1081 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
1141 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
1201 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
1261 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
1321 catgccgtct gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
1381 gatgagtggc agggcggggc gtaaacgcgt ggatccggct tactaaaagc cagataacag
1441 tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga
1501 agtatgtcaa aaagaggtgt gctatgaagc agcgtattac agtgacagtt gacagcgaca
1561 gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat
1621 gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat
1681 ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga acagggactg
1741 gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg
1801 atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg
1861 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg
1921 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag
1981 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct
2041 ggggaatata aatgtcaggc tccgttatac acagccagtc tgcaggtcga ccatagtgac
2101 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
2161 atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggtgatc
2221 gattcgacag atcactgaaa tgtgtgggcg tggcttaagg gtgggaaaga atatataagg
2281 tgggggtctt atgtagtttt gtatctgttt tgcagcagcc gccgccgcca tgagcaccaa
2341 ctcgtttgat ggaagcattg tgagctcata tttgacaacg cgcatgcccc catgggccgg
2401 ggtgcgtcag aatgtgatgg gctccagcat tgatggtcgc cccgtcctgc ccgcaaactc
2461 tactaccttg acctacgaga ccgtgtctgg aacgccgttg gagactgcag cctccgccgc
2521 cgcttcagcc gctgcagcca ccgcccgcgg gattgtgact gactttgctt tcctgagccc
2581 gcttgcaagc agtgcagctt cccgttcatc cgcccgcgat gacaagttga cggctctttt
2641 ggcacaattg gattctttga cccgggaact taatgtcgtt tctcagcagc tgttggatct
2701 gcgccagcag gtttctgccc tgaaggcttc ctcccctccc aatgcggttt aaaacataaa
2761 taaaaaacca gactctgttt ggatttggat caagcaagtg tcttgctgtc tttatttagg
2821 ggttttgcgc gcgcggtagg cccgggacca gcggtctcgg tcgttgaggg tcctgtgtat
2881 tttttccagg acgtggtaaa ggtgactctg gatgttcaga tacatgggca taagcccgtc
2941 tctggggtgg aggtagcacc actgcagagc ttcatgctgc ggggtggtgt tgtagatgat
3001 ccagtcgtag caggagcgct gggcgtggtg cctaaaaatg tctttcagta gcaagctgat
3061 tgccaggggc aggcccttgg tgtaagtgtt tacaaagcgg ttaagctggg atgggtgcat
3121 acgtggggat atgagatgca tcttggactg tatttttagg ttggctatgt tcccagccat
3181 atccctccgg ggattcatgt tgtgcagaac caccagcaca gtgtatccgg tgcacttggg
3241 aaatttgtca tgtagcttag aaggaaatgc gtggaagaac ttggagacgc ccttgtgacc
3301 tccaagattt tccatgcatt cgtccataat gatggcaatg ggcccacggg cggcggcctg
3361 ggcgaagata tttctgggat cactaacgtc atagttgtgt tccaggatga gatcgtcata
3421 ggccattttt acaaagcgcg ggcggagggt gccagactgc ggtataatgg ttccatccgg
3481 cccaggggcg tagttaccct cacagatttg catttcccac gctttgagtt cagatggggg
3541 gatcatgtct acctgcgggg cgatgaagaa aacggtttcc ggggtagggg agatcagctg
3601 ggaagaaagc aggttcctga gcagctgcga cttaccgcag ccggtgggcc cgtaaatcac
3661 acctattacc gggtgcaact ggtagttaag agagctgcag ctgccgtcat ccctgagcag
3721 gggggccact tcgttaagca tgtccctgac tcgcatgttt tccctgacca aatccgccag
3781 aaggcgctcg ccgcccagcg atagcagttc ttgcaaggaa gcaaagtttt tcaacggttt
3841 gagaccgtcc gccgtaggca tgcttttgag cgtttgacca agcagttcca ggcggtccca
3901 cagctcggtc acctgctcta cggcatctcg atccagcata tctcctcgtt tcgcgggttg
3961 gggcggcttt cgctgtacgg cagtagtcgg tgctcgtcca gacgggccag ggtcatgtct
4021 ttccacgggc gcagggtcct cgtcagcgta gtctgggtca cggtgaaggg gtgcgctccg
4081 ggctgcgcgc tggccagggt gcgcttgagg ctggtcctgc tggtgctgaa gcgctgccgg
4141 tcttcgccct gcgcgtcggc caggtagcat ttgaccatgg tgtcatagtc cagcccctcc
4201 gcggcgtggc ccttggcgcg cagcttgccc ttggaggagg cgccgcacga ggggcagtgc
4261 agacttttga gggcgtagag cttgggcgcg agaaataccg attccgggga gtaggcatcc
4321 gcgccgcagg ccccgcagac ggtctcgcat tccacgagcc aggtgagctc tggccgttcg
4381 gggtcaaaaa ccaggtttcc cccatgcttt ttgatgcgtt tcttacctct ggtttccatg
4441 agccggtgtc cacgctcggt gacgaaaagg ctgtccgtgt ccccgtatac agacttgaga
4501 ggcctgtcct cgagcggtgt tccgcggtcc tcctcgtata gaaactcgga ccactctgag
4561 acaaaggctc gcgtccaggc cagcacgaag gaggctaagt gggaggggta gcggtcgttg
4621 tccactaggg ggtccactcg ctccagggtg tgaagacaca tgtcgccctc ttcggcatca
4681 aggaaggtga ttggtttgta ggtgtaggcc acgtgaccgg gtgttcctga aggggggcta
4741 taaaaggggg tgggggcgcg ttcgtcctca ctctcttccg catcgctgtc tgcgagggcc
4801 agctgttggg gtgagtactc cctctgaaaa gcgggcatga cttctgcgct aagattgtca
4861 gtttccaaaa acgaggagga tttgatattc acctggcccg cggtgatgcc tttgagggtg
4921 gccgcatcca tctggtcaga aaagacaatc tttttgttgt caagcttggt ggcaaacgac
4981 ccgtagaggg cgttggacag caacttggcg atggagcgca gggtttggtt tttgtcgcga
5041 tcggcgcgct ccttggccgc gatgtttagc tgcacgtatt cgcgcgcaac gcaccgccat
5101 tcgggaaaga cggtggtgcg ctcgtcgggc accaggtgca cgcgccaacc gcggttgtgc
5161 agggtgacaa ggtcaacgct ggtggctacc tctccgcgta ggcgctcgtt ggtccagcag
5221 aggcggccgc ccttgcgcga gcagaatggc ggtagggggt ctagctgcgt ctcgtccggg
5281 gggtctgcgt ccacggtaaa gaccccgggc agcaggcgcg cgtcgaagta gtctatcttg
5341 catccttgca agtctagcgc ctgctgccat gcgcgggcgg caagcgcgcg ctcgtatggg
5401 ttgagtgggg gaccccatgg catggggtgg gtgagcgcgg aggcgtacat gccgcaaatg
5461 tcgtaaacgt agaggggctc tctgagtatt ccaagatatg tagggtagca tcttccaccg
5521 cggatgctgg cgcgcacgta atcgtatagt tcgtgcgagg gagcgaggag gtcgggaccg
5581 aggttgctac gggcgggctg ctctgctcgg aagactatct gcctgaagat ggcatgtgag
5641 ttggatgata tggttggacg ctggaagacg ttgaagctgg cgtctgtgag acctaccgcg
5701 tcacgcacga aggaggcgta ggagtcgcgc agcttgttga ccagctcggc ggtgacctgc
5761 acgtctaggg cgcagtagtc cagggtttcc ttgatgatgt catacttatc ctgtcccttt
5821 tttttccaca gctcgcggtt gaggacaaac tcttcgcggt ctttccagta ctcttggatc
5881 ggaaacccgt cggcctccga acggtaagag cctagcatgt agaactggtt gacggcctgg
5941 taggcgcagc atcccttttc tacgggtagc gcgtatgcct gcgcggcctt ccggagcgag
6001 gtgtgggtga gcgcaaaggt gtccctgacc atgactttga ggtactggta tttgaagtca
6061 gtgtcgtcgc atccgccctg ctcccagagc aaaaagtccg tgcgcttttt ggaacgcgga
6121 tttggcaggg cgaaggtgac atcgttgaag agtatctttc ccgcgcgagg cataaagttg
6181 cgtgtgatgc ggaagggtcc cggcacctcg gaacggttgt taattacctg ggcggcgagc
6241 acgatctcgt caaagccgtt gatgttgtgg cccacaatgt aaagttccaa gaagcgcggg
6301 atgcccttga tggaaggcaa ttttttaagt tcctcgtagg tgagctcttc aggggagctg
6361 agcccgtgct ctgaaagggc ccagtctgca agatgagggt tggaagcgac gaatgagctc
6421 cacaggtcac gggccattag catttgcagg tggtcgcgaa aggtcctaaa ctggcgacct
6481 atggccattt tttctggggt gatgcagtag aaggtaagcg ggtcttgttc ccagcggtcc
6541 catccaaggt tcgcggctag gtctcgcgcg gcagtcacta gaggctcatc tccgccgaac
6601 ttcatgacca gcatgaaggg cacgagctgc ttcccaaagg cccccatcca agtataggtc
6661 tctacatcgt aggtgacaaa gagacgctcg gtgcgaggat gcgagccgat cgggaagaac
6721 tggatctccc gccaccaatt ggaggagtgg ctattgatgt ggtgaaagta gaagtccctg
6781 cgacgggccg aacactcgtg ctggcttttg taaaaacgtg cgcagtactg gcagcggtgc
6841 acgggctgta catcctgcac gaggttgacc tgacgaccgc gcacaaggaa gcagagtggg
6901 aatttgagcc cctcgcctgg cgggtttggc tggtggtctt ctacttcggc tgcttgtcct
6961 tgaccgtctg gctgctcgag gggagttacg gtggatcgga ccaccacgcc gcgcgagccc
7021 aaagtccaga tgtccgcgcg cggcggtcgg agcttgatga caacatcgcg cagatgggag
7081 ctgtccatgg tctggagctc ccgcggcgtc aggtcaggcg ggagctcctg caggtttacc
7141 tcgcatagac gggtcagggc gcgggctaga tccaggtgat acctaatttc caggggctgg
7201 ttggtggcgg cgtcgatggc ttgcaagagg ccgcatcccc gcggcgcgac tacggtaccg
7261 cgcggcgggc ggtgggccgc gggggtgtcc ttggatgatg catctaaaag cggtgacgcg
7321 ggcgagcccc cggaggtagg gggggctccg gacccgccgg gagagggggc aggggcacgt
7381 cggcgccgcg cgcgggcagg agctggtgct gcgcgcgtag gttgctggcg aacgcgacga
7441 cgcggcggtt gatctcctga atctggcgcc tctgcgtgaa gacgacgggc ccggtgagct
7501 tgagcctgaa agagagttcg acagaatcaa tttcggtgtc gttgacggcg gcctggcgca
7561 aaatctcctg cacgtctcct gagttgtctt gataggcgat ctcggccatg aactgctcga
7621 tctcttcctc ctggagatct ccgcgtccgg ctcgctccac ggtggcggcg aggtcgttgg
7681 aaatgcgggc catgagctgc gagaaggcgt tgaggcctcc ctcgttccag acgcggctgt
7741 agaccacgcc cccttcggca tcgcgggcgc gcatgaccac ctgcgcgaga ttgagctcca
7801 cgtgccgggc gaagacggcg tagtttcgca ggcgctgaaa gaggtagttg agggtggtgg
7861 cggtgtgttc tgccacgaag aagtacataa cccagcgtcg caacgtggat tcgttgatat
7921 cccccaaggc ctcaaggcgc tccatggcct cgtagaagtc cacggcgaag ttgaaaaact
7981 gggagttgcg cgccgacacg gttaactcct cctccagaag acggatgagc tcggcgacag
8041 tgtcgcgcac ctcgcgctca aaggctacag gggcctcttc ttcttcttca atctcctctt
8101 ccataagggc ctccccttct tcttcttctg gcggcggtgg gggagggggg acacggcggc
8161 gacgacggcg caccgggagg cggtcgacaa agcgctcgat catctccccg cggcgacggc
8221 gcatggtctc ggtgacggcg cggccgttct cgcgggggcg cagttggaag acgccgcccg
8281 tcatgtcccg gttatgggtt ggcggggggc tgccatgcgg cagggatacg gcgctaacga
8341 tgcatctcaa caattgttgt gtaggtactc cgccgccgag ggacctgagc gagtccgcat
8401 cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg caaggtaggc
8461 tgagcaccgt ggcgggcggc agcgggcggc ggtcggggtt gtttctggcg gaggtgctgc
8521 tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgacaga agcaccatgt
8581 ccttgggtcc ggcctgctga atgcgcaggc ggtcggccat gccccaggct tcgttttgac
8641 atcggcgcag gtctttgtag tagtcttgca tgagcctttc taccggcact tcttcttctc
8701 cttcctcttg tcctgcatct cttgcatcta tcgctgcggc ggcggcggag tttggccgta
8761 ggtggcgccc tcttcctccc atgcgtgtga ccccgaagcc cctcatcggc tgaagcaggg
8821 ctaggtcggc gacaacgcgc tcggctaata tggcctgctg cacctgcgtg agggtagact
8881 ggaagtcatc catgtccaca aagcggtggt atgcgcccgt gttgatggtg taagtgcagt
8941 tggccataac ggaccagtta acggtctggt gacccggctg cgagagctcg gtgtacctga
9001 gacgcgagta agccctcgag tcaaatacgt agtcgttgca agtccgcacc aggtactggt
9061 atcccaccaa aaagtgcggc ggcggctggc ggtagagggg ccagcgtagg gtggccgggg
9121 ctccgggggc gagatcttcc aacataaggc gatgatatcc gtagatgtac ctggacatcc
9181 aggtgatgcc ggcggcggtg gtggaggcgc gcggaaagtc gcggacgcgg ttccagatgt
9241 tgcgcagcgg caaaaagtgc tccatggtcg ggacgctctg gccggtcagg cgcgcgcaat
9301 cgttgacgct ctagaccgtg caaaaggaga gcctgtaagc gggcactctt ccgtggtctg
9361 gtggataaat tcgcaagggt atcatggcgg acgaccgggg ttcgagcccc gtatccggcc
9421 gtccgccgtg atccatgcgg ttaccgcccg cgtgtcgaac ccaggtgtgc gacgtcagac
9481 aacgggggag tgctcctttt ggcttccttc caggcgcggc ggctgctgcg ctagcttttt
9541 tggccactgg ccgcgcgcag cgtaagcggt taggctggaa agcgaaagca ttaagtggct
9601 cgctccctgt agccggaggg ttattttcca agggttgagt cgcgggaccc ccggttcgag
9661 tctcggaccg gccggactgc ggcgaacggg ggtttgcctc cccgtcatgc aagaccccgc
9721 ttgcaaattc ctccggaaac agggacgagc cccttttttg cttttcccag atgcatccgg
9781 tgctgcggca gatgcgcccc cctcctcagc agcggcaaga gcaagagcag cggcagacat
9841 gcagggcacc ctcccctcct cctaccgcgt caggaggggc gacatccgcg gttgacgcgg
9901 cagcagatgg tgattacgaa cccccgcggc gccgggcccg gcactacctg gacttggagg
9961 agggcgaggg cctggcgcgg ctaggagcgc cctctcctga gcggtaccca agggtgcagc
10021 tgaagcgtga tacgcgtgag gcgtacgtgc cgcggcagaa cctgtttcgc gaccgcgagg
10081 gagaggagcc cgaggagatg cgggatcgaa agttccacgc agggcgcgag ctgcggcatg
10141 gcctgaatcg cgagcggttg ctgcgcgagg aggactttga gcccgacgcg cgaaccggga
10201 ttagtcccgc gcgcgcacac gtggcggccg ccgacctggt aaccgcatac gagcagacgg
10261 tgaaccagga gattaacttt caaaaaagct ttaacaacca cgtgcgtacg cttgtggcgc
10321 gcgaggaggt ggctatagga ctgatgcatc tgtgggactt tgtaagcgcg ctggagcaaa
10381 acccaaatag caagccgctc atggcgcagc tgttccttat agtgcagcac agcagggaca
10441 acgaggcatt cagggatgcg ctgctaaaca tagtagagcc cgagggccgc tggctgctcg
10501 atttgataaa catcctgcag agcatagtgg tgcaggagcg cagcttgagc ctggctgaca
10561 aggtggccgc catcaactat tccatgctta gcctgggcaa gttttacgcc cgcaagatat
10621 accatacccc ttacgttccc atagacaagg aggtaaagat cgaggggttc tacatgcgca
10681 tggcgctgaa ggtgcttacc ttgagcgacg acctgggcgt ttatcgcaac gagcgcatcc
10741 acaaggccgt gagcgtgagc cggcggcgcg agctcagcga ccgcgagctg atgcacagcc
10801 tgcaaagggc cctggctggc acgggcagcg gcgatagaga ggccgagtcc tactttgacg
10861 cgggcgctga cctgcgctgg gccccaagcc gacgcgccct ggaggcagct ggggccggac
10921 ctgggctggc ggtggcaccc gcgcgcgctg gcaacgtcgg cggcgtggag gaatatgacg
10981 aggacgatga gtacgagcca gaggacggcg agtactaagc ggtgatgttt ctgatcagat
11041 gatgcaagac gcaacggacc cggcggtgcg ggcggcgctg cagagccagc cgtccggcct
11101 taactccacg gacgactggc gccaggtcat ggaccgcatc atgtcgctga ctgcgcgcaa
11161 tcctgacgcg ttccggcagc agccgcaggc caaccggctc tccgcaattc tggaagcggt
11221 ggtcccggcg cgcgcaaacc ccacgcacga gaaggtgctg gcgatcgtaa acgcgctggc
11281 cgaaaacagg gccatccggc ccgacgaggc cggcctggtc tacgacgcgc tgcttcagcg
11341 cgtggctcgt tacaacagcg gcaacgtgca gaccaacctg gaccggctgg tgggggatgt
11401 gcgcgaggcc gtggcgcagc gtgagcgcgc gcagcagcag ggcaacctgg gctccatggt
11461 tgcactaaac gccttcctga gtacacagcc cgccaacgtg ccgcggggac aggaggacta
11521 caccaacttt gtgagcgcac tgcggctaat ggtgactgag acaccgcaaa gtgaggtgta
11581 ccagtctggg ccagactatt ttttccagac cagtagacaa ggcctgcaga ccgtaaacct
11641 gagccaggct ttcaaaaact tgcaggggct gtggggggtg cgggctccca caggcgaccg
11701 cgcgaccgtg tctagcttgc tgacgcccaa ctcgcgcctg ttgctgctgc taatagcgcc
11761 cttcacggac agtggcagcg tgtcccggga cacataccta ggtcacttgc tgacactgta
11821 ccgcgaggcc ataggtcagg cgcatgtgga cgagcatact ttccaggaga ttacaagtgt
11881 cagccgcgcg ctggggcagg aggacacggg cagcctggag gcaaccctaa actacctgct
11941 gaccaaccgg cggcagaaga tcccctcgtt gcacagttta aacagcgagg aggagcgcat
12001 tttgcgctac gtgcagcaga gcgtgagcct taacctgatg cgcgacgggg taacgcccag
12061 cgtggcgctg gacatgaccg cgcgcaacat ggaaccgggc atgtatgcct caaaccggcc
12121 gtttatcaac cgcctaatgg actacttgca tcgcgcggcc gccgtgaacc ccgagtattt
12181 caccaatgcc atcttgaacc cgcactggct accgccccct ggtttctaca ccgggggatt
12241 cgaggtgccc gagggtaacg atggattcct ctgggacgac atagacgaca gcgtgttttc
12301 cccgcaaccg cagaccctgc tagagttgca acagcgcgag caggcagagg cggcgctgcg
12361 aaaggaaagc ttccgcaggc caagcagctt gtccgatcta ggcgctgcgg ccccgcggtc
12421 agatgctagt agcccatttc caagcttgat agggtctctt accagcactc gcaccacccg
12481 cccgcgcctg ctgggcgagg aggagtacct aaacaactcg ctgctgcagc cgcagcgcga
12541 aaaaaacctg cctccggcat ttcccaacaa cgggatagag agcctagtgg acaagatgag
12601 tagatggaag acgtacgcgc aggagcacag ggacgtgcca ggcccgcgcc cgcccacccg
12661 tcgtcaaagg cacgaccgtc agcggggtct ggtgtgggag gacgatgact cggcagacga
12721 cagcagcgtc ctggatttgg gagggagtgg caacccgttt gcgcaccttc gccccaggct
12781 ggggagaatg ttttaaaaaa aaaaaagcat gatgcaaaat aaaaaactca ccaaggccat
12841 ggcaccgagc gttggttttc ttgtattccc cttagtatgc ggcgcgcggc gatgtatgag
12901 gaaggtcctc ctccctccta cgagagtgtg gtgagcgcgg cgccagtggc ggcggcgctg
12961 ggttctccct tcgatgctcc cctggacccg ccgtttgtgc ctccgcggta cctgcggcct
13021 accgggggga gaaacagcat ccgttactct gagttggcac ccctattcga caccacccgt
13081 gtgtacctgg tggacaacaa gtcaacggat gtggcatccc tgaactacca gaacgaccac
13141 agcaactttc tgaccacggt cattcaaaac aatgactaca gcccggggga ggcaagcaca
13201 cagaccatca atcttgacga ccggtcgcac tggggcggcg acctgaaaac catcctgcat
13261 accaacatgc caaatgtgaa cgagttcatg tttaccaata agtttaaggc gcgggtgatg
13321 gtgtcgcgct tgcctactaa ggacaatcag gtggagctga aatacgagtg ggtggagttc
13381 acgctgcccg agggcaacta ctccgagacc atgaccatag accttatgaa caacgcgatc
13441 gtggagcact acttgaaagt gggcagacag aacggggttc tggaaagcga catcggggta
13501 aagtttgaca cccgcaactt cagactgggg tttgaccccg tcactggtct tgtcatgcct
13561 ggggtatata caaacgaagc cttccatcca gacatcattt tgctgccagg atgcggggtg
13621 gacttcaccc acagccgcct gagcaacttg ttgggcatcc gcaagcggca acccttccag
13681 gagggcttta ggatcaccta cgatgatctg gagggtggta acattcccgc actgttggat
13741 gtggacgcct accaggcgag cttgaaagat gacaccgaac agggcggggg tggcgcaggc
13801 ggcagcaaca gcagtggcag cggcgcggaa gagaactcca acgcggcagc cgcggcaatg
13861 cagccggtgg aggacatgaa cgatcatgcc attcgcggcg acacctttgc cacacgggct
13921 gaggagaagc gcgctgaggc cgaagcagcg gccgaagctg ccgcccccgc tgcgcaaccc
13981 gaggtcgaga agcctcagaa gaaaccggtg atcaaacccc tgacagagga cagcaagaaa
14041 cgcagttaca acctaataag caatgacagc accttcaccc agtaccgcag ctggtacctt
14101 gcatacaact acggcgaccc tcagaccgga atccgctcat ggaccctgct ttgcactcct
14161 gacgtaacct gcggctcgga gcaggtctac tggtcgttgc cagacatgat gcaagacccc
14221 gtgaccttcc gctccacgcg ccagatcagc aactttccgg tggtgggcgc cgagctgttg
14281 cccgtgcact ccaagagctt ctacaacgac caggccgtct actcccaact catccgccag
14341 tttacctctc tgacccacgt gttcaatcgc tttcccgaga accagatttt ggcgcgcccg
14401 ccagccccca ccatcaccac cgtcagtgaa aacgttcctg ctctcacaga tcacgggacg
14461 ctaccgctgc gcaacagcat cggaggagtc cagcgagtga ccattactga cgccagacgc
14521 cgcacctgcc cctacgttta caaggccctg ggcatagtct cgccgcgcgt cctatcgagc
14581 cgcacttttt gagcaagcat gtccatcctt atatcgccca gcaataacac aggctggggc
14641 ctgcgcttcc caagcaagat gtttggcggg gccaagaagc gctccgacca acacccagtg
14701 cgcgtgcgcg ggcactaccg cgcgccctgg ggcgcgcaca aacgcggccg cactgggcgc
14761 accaccgtcg atgacgccat cgacgcggtg gtggaggagg cgcgcaacta cacgcccacg
14821 ccgccaccag tgtccacagt ggacgcggcc attcagaccg tggtgcgcgg agcccggcgc
14881 tatgctaaaa tgaagagacg gcggaggcgc gtagcacgtc gccaccgccg ccgacccggc
14941 actgccgccc aacgcgcggc ggcggccctg cttaaccgcg cacgtcgcac cggccgacgg
15001 gcggccatgc gggccgctcg aaggctggcc gcgggtattg tcactgtgcc ccccaggtcc
15061 aggcgacgag cggccgccgc agcagccgcg gccattagtg ctatgactca gggtcgcagg
15121 ggcaacgtgt attgggtgcg cgactcggtt agcggcctgc gcgtgcccgt gcgcacccgc
15181 cccccgcgca actagattgc aagaaaaaac tacttagact cgtactgttg tatgtatcca
15241 gcggcggcgg cgcgcaacga agctatgtcc aagcgcaaaa tcaaagaaga gatgctccag
15301 gtcatcgcgc cggagatcta tggccccccg aagaaggaag agcaggatta caagccccga
15361 aagctaaagc gggtcaaaaa gaaaaagaaa gatgatgatg atgaacttga cgacgaggtg
15421 gaactgctgc acgctaccgc gcccaggcga cgggtacagt ggaaaggtcg acgcgtaaaa
15481 cgtgttttgc gacccggcac caccgtagtc tttacgcccg gtgagcgctc cacccgcacc
15541 tacaagcgcg tgtatgatga ggtgtacggc gacgaggacc tgcttgagca ggccaacgag
15601 cgcctcgggg agtttgccta cggaaagcgg cataaggaca tgctggcgtt gccgctggac
15661 gagggcaacc caacacctag cctaaagccc gtaacactgc agcaggtgct gcccgcgctt
15721 gcaccgtccg aagaaaagcg cggcctaaag cgcgagtctg gtgacttggc acccaccgtg
15781 cagctgatgg tacccaagcg ccagcgactg gaagatgtct tggaaaaaat gaccgtggaa
15841 cctgggctgg agcccgaggt ccgcgtgcgg ccaatcaagc aggtggcgcc gggactgggc
15901 gtgcagaccg tggacgttca gatacccact accagtagca ccagtattgc caccgccaca
15961 gagggcatgg agacacaaac gtccccggtt gcctcagcgg tggcggatgc cgcggtgcag
16021 gcggtcgctg cggccgcgtc caagacctct acggaggtgc aaacggaccc gtggatgttt
16081 cgcgtttcag ccccccggcg cccgcgcggt tcgaggaagt acggcgccgc cagcgcgcta
16141 ctgcccgaat atgccctaca tccttccatt gcgcctaccc ccggctatcg tggctacacc
16201 taccgcccca gaagacgagc aactacccga cgccgaacca ccactggaac ccgccgccgc
16261 cgtcgccgtc gccagcccgt gctggccccg atttccgtgc gcagggtggc tcgcgaagga
16321 ggcaggaccc tggtgctgcc aacagcgcgc taccacccca gcatcgttta aaagccggtc
16381 tttgtggttc ttgcagatat ggccctcacc tgccgcctcc gtttcccggt gccgggattc
16441 cgaggaagaa tgcaccgtag gaggggcatg gccggccacg gcctgacggg cggcatgcgt
16501 cgtgcgcacc accggcggcg gcgcgcgtcg caccgtcgca tgcgcggcgg tatcctgccc
16561 ctccttattc cactgatcgc cgcggcgatt ggcgccgtgc ccggaattgc atccgtggcc
16621 ttgcaggcgc agagacactg attaaaaaca agttgcatgt ggaaaaatca aaataaaaag
16681 tctggactct cacgctcgct tggtcctgta actattttgt agaatggaag acatcaactt
16741 tgcgtctctg gccccgcgac acggctcgcg cccgttcatg ggaaactggc aagatatcgg
16801 caccagcaat atgagcggtg gcgccttcag ctggggctcg ctgtggagcg gcattaaaaa
16861 tttcggttcc accgttaaga actatggcag caaggcctgg aacagcagca caggccagat
16921 gctgagggat aagttgaaag agcaaaattt ccaacaaaag gtggtagatg gcctggcctc
16981 tggcattagc ggggtggtgg acctggccaa ccaggcagtg caaaataaga ttaacagtaa
17041 gcttgatccc cgccctcccg tagaggagcc tccaccggcc gtggagacag tgtctccaga
17101 ggggcgtggc gaaaagcgtc cgcgccccga cagggaagaa actctggtga cgcaaataga
17161 cgagcctccc tcgtacgagg aggcactaaa gcaaggcctg cccaccaccc gtcccatcgc
17221 gcccatggct accggagtgc tgggccagca cacacccgta acgctggacc tgcctccccc
17281 cgccgacacc cagcagaaac ctgtgctgcc aggcccgacc gccgttgttg taacccgtcc
17341 tagccgcgcg tccctgcgcc gcgccgccag cggtccgcga tcgttgcggc ccgtagccag
17401 tggcaactgg caaagcacac tgaacagcat cgtgggtctg ggggtgcaat ccctgaagcg
17461 ccgacgatgc ttctgaatag ctaacgtgtc gtatgtgtgt catgtatgcg tccatgtcgc
17521 cgccagagga gctgctgagc cgccgcgcgc ccgctttcca agatggctac cccttcgatg
17581 atgccgcagt ggtcttacat gcacatctcg ggccaggacg cctcggagta cctgagcccc
17641 gggctggtgc agtttgcccg cgccaccgag acgtacttca gcctgaataa caagtttaga
17701 aaccccacgg tggcgcctac gcacgacgtg accacagacc ggtcccagcg tttgacgctg
17761 cggttcatcc ctgtggaccg tgaggatact gcgtactcgt acaaggcgcg gttcacccta
17821 gctgtgggtg ataaccgtgt gctggacatg gcttccacgt actttgacat ccgcggcgtg
17881 ctggacaggg gccctacttt taagccctac tctggcactg cctacaacgc cctggctccc
17941 aagggtgccc caaatccttg cgaatgggat gaagctgcta ctgctcttga aataaaccta
18001 gaagaagagg acgatgacaa cgaagacgaa gtagacgagc aagctgagca gcaaaaaact
18061 cacgtatttg ggcaggcgcc ttattctggt ataaatatta caaaggaggg tattcaaata
18121 ggtgtcgaag gtcaaacacc taaatatgcc gataaaacat ttcaacctga acctcaaata
18181 ggagaatctc agtggtacga aactgaaatt aatcatgcag ctgggagagt ccttaaaaag
18241 actaccccaa tgaaaccatg ttacggttca tatgcaaaac ccacaaatga aaatggaggg
18301 caaggcattc ttgtaaagca acaaaatgga aagctagaaa gtcaagtgga aatgcaattt
18361 ttctcaacta ctgaggcgac cgcaggcaat ggtgataact tgactcctaa agtggtattg
18421 tacagtgaag atgtagatat agaaacccca gacactcata tttcttacat gcccactatt
18481 aaggaaggta actcacgaga actaatgggc caacaatcta tgcccaacag gcctaattac
18541 attgctttta gggacaattt tattggtcta atgtattaca acagcacggg taatatgggt
18601 gttctggcgg gccaagcatc gcagttgaat gctgttgtag atttgcaaga cagaaacaca
18661 gagctttcat accagctttt gcttgattcc attggtgata gaaccaggta cttttctatg
18721 tggaatcagg ctgttgacag ctatgatcca gatgttagaa ttattgaaaa tcatggaact
18781 gaagatgaac ttccaaatta ctgctttcca ctgggaggtg tgattaatac agagactctt
18841 accaaggtaa aacctaaaac aggtcaggaa aatggatggg aaaaagatgc tacagaattt
18901 tcagataaaa atgaaataag agttggaaat aattttgcca tggaaatcaa tctaaatgcc
18961 aacctgtgga gaaatttcct gtactccaac atagcgctgt atttgcccga caagctaaag
19021 tacagtcctt ccaacgtaaa aatttctgat aacccaaaca cctacgacta catgaacaag
19081 cgagtggtgg ctcccgggtt agtggactgc tacattaacc ttggagcacg ctggtccctt
19141 gactatatgg acaacgtcaa cccatttaac caccaccgca atgctggcct gcgctaccgc
19201 tcaatgttgc tgggcaatgg tcgctatgtg cccttccaca tccaggtgcc tcagaagttc
19261 tttgccatta aaaacctcct tctcctgccg ggctcataca cctacgagtg gaacttcagg
19321 aaggatgtta acatggttct gcagagctcc ctaggaaatg acctaagggt tgacggagcc
19381 agcattaagt ttgatagcat ttgcctttac gccaccttct tccccatggc ccacaacacc
19441 gcctccacgc ttgaggccat gcttagaaac gacaccaacg accagtcctt taacgactat
19501 ctctccgccg ccaacatgct ctaccctata cccgccaacg ctaccaacgt gcccatatcc
19561 atcccctccc gcaactgggc ggctttccgc ggctgggcct tcacgcgcct taagactaag
19621 gaaaccccat cactgggctc gggctacgac ccttattaca cctactctgg ctctataccc
19681 tacctagatg gaacctttta cctcaaccac acctttaaga aggtggccat tacctttgac
19741 tcttctgtca gctggcctgg caatgaccgc ctgcttaccc ccaacgagtt tgaaattaag
19801 cgctcagttg acggggaggg ttacaacgtt gcccagtgta acatgaccaa agactggttc
19861 ctggtacaaa tgctagctaa ctacaacatt ggctaccagg gcttctatat cccagagagc
19921 tacaaggacc gcatgtactc cttctttaga aacttccagc ccatgagccg tcaggtggtg
19981 gatgatacta aatacaagga ctaccaacag gtgggcatcc tacaccaaca caacaactct
20041 ggatttgttg gctaccttgc ccccaccatg cgcgaaggac aggcctaccc tgctaacttc
20101 ccctatccgc ttataggcaa gaccgcagtt gacagcatta cccagaaaaa gtttctttgc
20161 gatcgcaccc tttggcgcat cccattctcc agtaacttta tgtccatggg cgcactcaca
20221 gacctgggcc aaaaccttct ctacgccaac tccgcccacg cgctagacat gacttttgag
20281 gtggatccca tggacgagcc cacccttctt tatgttttgt ttgaagtctt tgacgtggtc
20341 cgtgtgcacc ggccgcaccg cggcgtcatc gaaaccgtgt acctgcgcac gcccttctcg
20401 gccggcaacg ccacaacata aagaagcaag caacatcaac aacagctgcc gccatgggct
20461 ccagtgagca ggaactgaaa gccattgtca aagatcttgg ttgtgggcca tattttttgg
20521 gcacctatga caagcgcttt ccaggctttg tttctccaca caagctcgcc tgcgccatag
20581 tcaatacggc cggtcgcgag actgggggcg tacactggat ggcctttgcc tggaacccgc
20641 actcaaaaac atgctacctc tttgagccct ttggcttttc tgaccagcga ctcaagcagg
20701 tttaccagtt tgagtacgag tcactcctgc gccgtagcgc cattgcttct tcccccgacc
20761 gctgtataac gctggaaaag tccacccaaa gcgtacaggg gcccaactcg gccgcctgtg
20821 gactattctg ctgcatgttt ctccacgcct ttgccaactg gccccaaact cccatggatc
20881 acaaccccac catgaacctt attaccgggg tacccaactc catgctcaac agtccccagg
20941 tacagcccac cctgcgtcgc aaccaggaac agctctacag cttcctggag cgccactcgc
21001 cctacttccg cagccacagt gcgcagatta ggagcgccac ttctttttgt cacttgaaaa
21061 acatgtaaaa ataatgtact agagacactt tcaataaagg caaatgcttt tatttgtaca
21121 ctctcgggtg attatttacc cccacccttg ccgtctgcgc cgtttaaaaa tcaaaggggt
21181 tctgccgcgc atcgctatgc gccactggca gggacacgtt gcgatactgg tgtttagtgc
21241 tccacttaaa ctcaggcaca accatccgcg gcagctcggt gaagttttca ctccacaggc
21301 tgcgcaccat caccaacgcg tttagcaggt cgggcgccga tatcttgaag tcgcagttgg
21361 ggcctccgcc ctgcgcgcgc gagttgcgat acacagggtt gcagcactgg aacactatca
21421 gcgccgggtg gtgcacgctg gccagcacgc tcttgtcgga gatcagatcc gcgtccaggt
21481 cctccgcgtt gctcagggcg aacggagtca actttggtag ctgccttccc aaaaagggcg
21541 cgtgcccagg ctttgagttg cactcgcacc gtagtggcat caaaaggtga ccgtgcccgg
21601 tctgggcgtt aggatacagc gcctgcataa aagccttgat ctgcttaaaa gccacctgag
21661 cctttgcgcc ttcagagaag aacatgccgc aagacttgcc ggaaaactga ttggccggac
21721 aggccgcgtc gtgcacgcag caccttgcgt cggtgttgga gatctgcacc acatttcggc
21781 cccaccggtt cttcacgatc ttggccttgc tagactgctc cttcagcgcg cgctgcccgt
21841 tttcgctcgt cacatccatt tcaatcacgt gctccttatt tatcataatg cttccgtgta
21901 gacacttaag ctcgccttcg atctcagcgc agcggtgcag ccacaacgcg cagcccgtgg
21961 gctcgtgatg cttgtaggtc acctctgcaa acgactgcag gtacgcctgc aggaatcgcc
22021 ccatcatcgt cacaaaggtc ttgttgctgg tgaaggtcag ctgcaacccg cggtgctcct
22081 cgttcagcca ggtcttgcat acggccgcca gagcttccac ttggtcaggc agtagtttga
22141 agttcgcctt tagatcgtta tccacgtggt acttgtccat cagcgcgcgc gcagcctcca
22201 tgcccttctc ccacgcagac acgatcggca cactcagcgg gttcatcacc gtaatttcac
22261 tttccgcttc gctgggctct tcctcttcct cttgcgtccg cataccacgc gccactgggt
22321 cgtcttcatt cagccgccgc actgtgcgct tacctccttt gccatgcttg attagcaccg
22381 gtgggttgct gaaacccacc atttgtagcg ccacatcttc tctttcttcc tcgctgtcca
22441 cgattacctc tggtgatggc gggcgctcgg gcttgggaga agggcgcttc tttttcttct
22501 tgggcgcaat ggccaaatcc gccgccgagg tcgatggccg cgggctgggt gtgcgcggca
22561 ccagcgcgtc ttgtgatgag tcttcctcgt cctcggactc gatacgccgc ctcatccgct
22621 tttttggggg cgcccgggga ggcggcggcg acggggacgg ggacgacacg tcctccatgg
22681 ttgggggacg tcgcgccgca ccgcgtccgc gctcgggggt ggtttcgcgc tgctcctctt
22741 cccgactggc catttccttc tcctataggc agaaaaagat catggagtca gtcgagaaga
22801 aggacagcct aaccgccccc tctgagttcg ccaccaccgc ctccaccgat gccgccaacg
22861 cgcctaccac cttccccgtc gaggcacccc cgcttgagga ggaggaagtg attatcgagc
22921 aggacccagg ttttgtaagc gaagacgacg aggaccgctc agtaccaaca gaggataaaa
22981 agcaagacca ggacaacgca gaggcaaacg aggaacaagt cgggcggggg gacgaaaggc
23041 atggcgacta cctagatgtg ggagacgacg tgctgttgaa gcatctgcag cgccagtgcg
23101 ccattatctg cgacgcgttg caagagcgca gcgatgtgcc cctcgccata gcggatgtca
23161 gccttgccta cgaacgccac ctattctcac cgcgcgtacc ccccaaacgc caagaaaacg
23221 gcacatgcga gcccaacccg cgcctcaact tctaccccgt atttgccgtg ccagaggtgc
23281 ttgccaccta tcacatcttt ttccaaaact gcaagatacc cctatcctgc cgtgccaacc
23341 gcagccgagc ggacaagcag ctggccttgc ggcagggcgc tgtcatacct gatatcgcct
23401 cgctcaacga agtgccaaaa atctttgagg gtcttggacg cgacgagaag cgcgcggcaa
23461 acgctctgca acaggaaaac agcgaaaatg aaagtcactc tggagtgttg gtggaactcg
23521 agggtgacaa cgcgcgccta gccgtactaa aacgcagcat cgaggtcacc cactttgcct
23581 acccggcact taacctaccc cccaaggtca tgagcacagt catgagtgag ctgatcgtgc
23641 gccgtgcgca gcccctggag agggatgcaa atttgcaaga acaaacagag gagggcctac
23701 ccgcagttgg cgacgagcag ctagcgcgct ggcttcaaac gcgcgagcct gccgacttgg
23761 aggagcgacg caaactaatg atggccgcag tgctcgttac cgtggagctt gagtgcatgc
23821 agcggttctt tgctgacccg gagatgcagc gcaagctaga ggaaacattg cactacacct
23881 ttcgacaggg ctacgtacgc caggcctgca agatctccaa cgtggagctc tgcaacctgg
23941 tctcctacct tggaattttg cacgaaaacc gccttgggca aaacgtgctt cattccacgc
24001 tcaagggcga ggcgcgccgc gactacgtcc gcgactgcgt ttacttattt ctatgctaca
24061 cctggcagac ggccatgggc gtttggcagc agtgcttgga ggagtgcaac ctcaaggagc
24121 tgcagaaact gctaaagcaa aacttgaagg acctatggac ggccttcaac gagcgctccg
24181 tggccgcgca cctggcggac atcattttcc ccgaacgcct gcttaaaacc ctgcaacagg
24241 gtctgccaga cttcaccagt caaagcatgt tgcagaactt taggaacttt atcctagagc
24301 gctcaggaat cttgcccgcc acctgctgtg cacttcctag cgactttgtg cccattaagt
24361 accgcgaatg ccctccgccg ctttggggcc actgctacct tctgcagcta gccaactacc
24421 ttgcctacca ctctgacata atggaagacg tgagcggtga cggtctactg gagtgtcact
24481 gtcgctgcaa cctatgcacc ccgcaccgct ccctggtttg caattcgcag ctgcttaacg
24541 aaagtcaaat tatcggtacc tttgagctgc agggtccctc gcctgacgaa aagtccgcgg
24601 ctccggggtt gaaactcact ccggggctgt ggacgtcggc ttaccttcgc aaatttgtac
24661 ctgaggacta ccacgcccac gagattaggt tctacgaaga ccaatcccgc ccgccaaatg
24721 cggagcttac cgcctgcgtc attacccagg gccacattct tggccaattg caagccatca
24781 acaaagcccg ccaagagttt ctgctacgaa agggacgggg ggtttacttg gacccccagt
24841 ccggcgagga gctcaaccca atccccccgc cgccgcagcc ctatcagcag cagccgcggg
24901 cccttgcttc ccaggatggc acccaaaaag aagctgcagc tgccgccgcc acccacggac
24961 gaggaggaat actgggacag tcaggcagag gaggttttgg acgaggagga ggaggacatg
25021 atggaagact gggagagcct agacgaggaa gcttccgagg tcgaagaggt gtcagacgaa
25081 acaccgtcac cctcggtcgc attcccctcg ccggcgcccc agaaatcggc aaccggttcc
25141 agcatggcta caacctccgc tcctcaggcg ccgccggcac tgcccgttcg ccgacccaac
25201 cgtagatggg acaccactgg aaccagggcc ggtaagtcca agcagccgcc gccgttagcc
25261 caagagcaac aacagcgcca aggctaccgc tcatggcgcg ggcacaagaa cgccatagtt
25321 gcttgcttgc aagactgtgg gggcaacatc tccttcgccc gccgctttct tctctaccat
25381 cacggcgtgg ccttcccccg taacatcctg cattactacc gtcatctcta cagcccatac
25441 tgcaccggcg gcagcggcag cggcagcaac agcagcggcc acacagaagc aaaggcgacc
25501 ggatagcaag actctgacaa agcccaagaa atccacagcg gcggcagcag caggaggagg
25561 agcgctgcgt ctggcgccca acgaacccgt atcgacccgc gagcttagaa acaggatttt
25621 tcccactctg tatgctatat ttcaacagag caggggccaa gaacaagagc tgaaaataaa
25681 aaacaggtct ctgcgatccc tcacccgcag ctgcctgtat cacaaaagcg aagatcagct
25741 tcggcgcacg ctggaagacg cggaggctct cttcagtaaa tactgcgcgc tgactcttaa
25801 ggactagttt cgcgcccttt ctcaaattta agcgcgaaaa ctacgtcatc tccagcggcc
25861 acacccggcg ccagcacctg tcgtcagcgc cattatgagc aaggaaattc ccacgcccta
25921 catgtggagt taccagccac aaatgggact tgcggctgga gctgcccaag actactcaac
25981 ccgaataaac tacatgagcg cgggacccca catgatatcc cgggtcaacg gaatccgcgc
26041 ccaccgaaac cgaattctct tggaacaggc ggctattacc accacacctc gtaataacct
26101 taatccccgt agttggcccg ctgccctggt gtaccaggaa agtcccgctc ccaccactgt
26161 ggtacttccc agagacgccc aggccgaagt tcagatgact aactcagggg cgcagcttgc
26221 gggcggcttt cgtcacaggg tgcggtcgcc cgggcagggt ataactcacc tgacaatcag
26281 agggcgaggt attcagctca acgacgagtc ggtgagctcc tcgcttggtc tccgtccgga
26341 cgggacattt cagatcggcg gcgccggccg tccttcattc acgcctcgtc aggcaatcct
26401 aactctgcag acctcgtcct ctgagccgcg ctctggaggc attggaactc tgcaatttat
26461 tgaggagttt gtgccatcgg tctactttaa ccccttctcg ggacctcccg gccactatcc
26521 ggatcaattt attcctaact ttgacgcggt aaaggactcg gcggacggct acgactgaat
26581 gttaagtgga gaggcagagc aactgcgcct gaaacacctg gtccactgtc gccgccacaa
26641 gtgctttgcc cgcgactccg gtgagttttg ctactttgaa ttgcccgagg atcatatcga
26701 gggcccggcg cacggcgtcc ggcttaccgc ccagggagag cttgcccgta gcctgattcg
26761 ggagtttacc cagcgccccc tgctagttga gcgggacagg ggaccctgtg ttctcactgt
26821 gatttgcaac tgtcctaacc ttggattaca tcaagatctt tgttgccatc tctgtgctga
26881 gtataataaa tacagaaatt aaaatatact ggggctccta tcgccatcct gtaaacgcca
26941 ccgtcttcac ccgcccaagc aaaccaaggc gaaccttacc tggtactttt aacatctctc
27001 cctctgtgat ttacaacagt ttcaacccag acggagtgag tctacgagag aacctctccg
27061 agctcagcta ctccatcaga aaaaacacca ccctccttac ctgccgggaa cgtacgagtg
27121 cgtcaccggc cgctgcacca cacctaccgc ctgaccgtaa accagacttt ttccggacag
27181 acctcaataa ctctgtttac cagaacagga ggtgagctta gaaaaccctt agggtattag
27241 gccaaaggcg cagctactgt ggggtttatg aacaattcaa gcaactctac gggctattct
27301 aattcaggtt tctctagaaa tggacggaat tattacagag cagcgcctgc tagaaagacg
27361 cagggcagcg gccgagcaac agcgcatgaa tcaagagctc caagacatgg ttaacttgca
27421 ccagtgcaaa aggggtatct tttgtctggt aaagcaggcc aaagtcacct acgacagtaa
27481 taccaccgga caccgcctta gctacaagtt gccaaccaag cgtcagaaat tggtggtcat
27541 ggtgggagaa aagcccatta ccataactca gcactcggta gaaaccgaag gctgcattca
27601 ctcaccttgt caaggacctg aggatctctg cacccttatt aagaccctgt gcggtctcaa
27661 agatcttatt ccctttaact aataaaaaaa aataataaag catcacttac ttaaaatcag
27721 ttagcaaatt tctgtccagt ttattcagca gcacctcctt gccctcctcc cagctctggt
27781 attgcagctt cctcctggct gcaaactttc tccacaatct aaatggaatg tcagtttcct
27841 cctgttcctg tccatccgca cccactatct tcatgttgtt gcagatgaag cgcgcaagac
27901 cgtctgaaga taccttcaac cccgtgtatc catatgacac ggaaaccggt cctccaactg
27961 tgccttttct tactcctccc tttgtatccc ccaatgggtt tcaagagagt ccccctgggg
28021 tactctcttt gcgcctatcc gaacctctag ttacctccaa tggcatgctt gcgctcaaaa
28081 tgggcaacgg cctctctctg gacgaggccg gcaaccttac ctcccaaaat gtaaccactg
28141 tgagcccacc tctcaaaaaa accaagtcaa acataaacct ggaaatatct gcacccctca
28201 cagttacctc agaagcccta actgtggctg ccgccgcacc tctaatggtc gcgggcaaca
28261 cactcaccat gcaatcacag gccccgctaa ccgtgcacga ctccaaactt agcattgcca
28321 cccaaggacc cctcacagtg tcagaaggaa agctagccct gcaaacatca ggccccctca
28381 ccaccaccga tagcagtacc cttactatca ctgcctcacc ccctctaact actgccactg
28441 gtagcttggg cattgacttg aaagagccca tttatacaca aaatggaaaa ctaggactaa
28501 agtacggggc tcctttgcat gtaacagacg acctaaacac tttgaccgta gcaactggtc
28561 caggtgtgac tattaataat acttccttgc aaactaaagt tactggagcc ttgggttttg
28621 attcacaagg caatatgcaa cttaatgtag caggaggact aaggattgat tctcaaaaca
28681 gacgccttat acttgatgtt agttatccgt ttgatgctca aaaccaacta aatctaagac
28741 taggacaggg ccctcttttt ataaactcag cccacaactt ggatattaac tacaacaaag
28801 gcctttactt gtttacagct tcaaacaatt ccaaaaagct tgaggttaac ctaagcactg
28861 ccaaggggtt gatgtttgac gctacagcca tagccattaa tgcaggagat gggcttgaat
28921 ttggttcacc taatgcacca aacacaaatc ccctcaaaac aaaaattggc catggcctag
28981 aatttgattc aaacaaggct atggttccta aactaggaac tggccttagt tttgacagca
29041 caggtgccat tacagtagga aacaaaaata atgataagct aactttgtgg accacaccag
29101 ctccatctcc taactgtaga ctaaatgcag agaaagatgc taaactcact ttggtcttaa
29161 caaaatgtgg cagtcaaata cttgctacag tttcagtttt ggctgttaaa ggcagtttgg
29221 ctccaatatc tggaacagtt caaagtgctc atcttattat aagatttgac gaaaatggag
29281 tgctactaaa caattccttc ctggacccag aatattggaa ctttagaaat ggagatctta
29341 ctgaaggcac agcctataca aacgctgttg gatttatgcc taacctatca gcttatccaa
29401 aatctcacgg taaaactgcc aaaagtaaca ttgtcagtca agtttactta aacggagaca
29461 aaactaaacc tgtaacacta accattacac taaacggtac acaggaaaca ggagacacaa
29521 ctccaagtgc atactctatg tcattttcat gggactggtc tggccacaac tacattaatg
29581 aaatatttgc cacatcctct tacacttttt catacattgc ccaagaataa agaatcgttt
29641 gtgttatgtt tcaacgtgtt tatttttcaa ttgcagaaaa tttcgaatca tttttcattc
29701 agtagtatag ccccaccacc acatagctta tacagatcac cgtaccttaa tcaaactcac
29761 agaaccctag tattcaacct gccacctccc tcccaacaca cagagtacac agtcctttct
29821 ccccggctgg ccttaaaaag catcatatca tgggtaacag acatattctt aggtgttata
29881 ttccacacgg tttcctgtcg agccaaacgc tcatcagtga tattaataaa ctccccgggc
29941 agctcactta agttcatgtc gctgtccagc tgctgagcca caggctgctg tccaacttgc
30001 ggttgcttaa cgggcggcga aggagaagtc cacgcctaca tgggggtaga gtcataatcg
30061 tgcatcagga tagggcggtg gtgctgcagc agcgcgcgaa taaactgctg ccgccgccgc
30121 tccgtcctgc aggaatacaa catggcagtg gtctcctcag cgatgattcg caccgcccgc
30181 agcataaggc gccttgtcct ccgggcacag cagcgcaccc tgatctcact taaatcagca
30241 cagtaactgc agcacagcac cacaatattg ttcaaaatcc cacagtgcaa ggcgctgtat
30301 ccaaagctca tggcggggac cacagaaccc acgtggccat cataccacaa gcgcaggtag
30361 attaagtggc gacccctcat aaacacgctg gacataaaca ttacctcttt tggcatgttg
30421 taattcacca cctcccggta ccatataaac ctctgattaa acatggcgcc atccaccacc
30481 atcctaaacc agctggccaa aacctgcccg ccggctatac actgcaggga accgggactg
30541 gaacaatgac agtggagagc ccaggactcg taaccatgga tcatcatgct cgtcatgata
30601 tcaatgttgg cacaacacag gcacacgtgc atacacttcc tcaggattac aagctcctcc
30661 cgcgttagaa ccatatccca gggaacaacc cattcctgaa tcagcgtaaa tcccacactg
30721 cagggaagac ctcgcacgta actcacgttg tgcattgtca aagtgttaca ttcgggcagc
30781 agcggatgat cctccagtat ggtagcgcgg gtttctgtct caaaaggagg tagacgatcc
30841 ctactgtacg gagtgcgccg agacaaccga gatcgtgttg gtcgtagtgt catgccaaat
30901 ggaacgccgg acgtagtcat atttcctgaa gcaaaaccag gtgcgggcgt gacaaacaga
30961 tctgcgtctc cggtctcgcc gcttagatcg ctctgtgtag tagttgtagt atatccactc
31021 tctcaaagca tccaggcgcc ccctggcttc gggttctatg taaactcctt catgcgccgc
31081 tgccctgata acatccacca ccgcagaata agccacaccc agccaaccta cacattcgtt
31141 ctgcgagtca cacacgggag gagcgggaag agctggaaga accatgtttt tttttttatt
31201 ccaaaagatt atccaaaacc tcaaaatgaa gatctattaa gtgaacgcgc tcccctccgg
31261 tggcgtggtc aaactctaca gccaaagaac agataatggc atttgtaaga tgttgcacaa
31321 tggcttccaa aaggcaaacg gccctcacgt ccaagtggac gtaaaggcta aacccttcag
31381 ggtgaatctc ctctataaac attccagcac cttcaaccat gcccaaataa ttctcatctc
31441 gccaccttct caatatatct ctaagcaaat cccgaatatt aagtccggcc attgtaaaaa
31501 tctgctccag agcgccctcc accttcagcc tcaagcagcg aatcatgatt gcaaaaattc
31561 aggttcctca cagacctgta taagattcaa aagcggaaca ttaacaaaaa taccgcgatc
31621 ccgtaggtcc cttcgcaggg ccagctgaac ataatcgtgc aggtctgcac ggaccagcgc
31681 ggccacttcc ccgccaggaa ccttgacaaa agaacccaca ctgattatga cacgcatact
31741 cggagctatg ctaaccagcg tagccccgat gtaagctttg ttgcatgggc ggcgatataa
31801 aatgcaaggt gctgctcaaa aaatcaggca aagcctcgcg caaaaaagaa agcacatcgt
31861 agtcatgctc atgcagataa aggcaggtaa gctccggaac caccacagaa aaagacacca
31921 tttttctctc aaacatgtct gcgggtttct gcataaacac aaaataaaat aacaaaaaaa
31981 catttaaaca ttagaagcct gtcttacaac aggaaaaaca acccttataa gcataagacg
32041 gactacggcc atgccggcgt gaccgtaaaa aaactggtca ccgtgattaa aaagcaccac
32101 cgacagctcc tcggtcatgt ccggagtcat aatgtaagac tcggtaaaca catcaggttg
32161 attcacatcg gtcagtgcta aaaagcgacc gaaatagccc gggggaatac atacccgcag
32221 gcgtagagac aacattacag cccccatagg aggtataaca aaattaatag gagagaaaaa
32281 cacataaaca cctgaaaaac cctcctgcct aggcaaaata gcaccctccc gctccagaac
32341 aacatacagc gcttccacag cggcagccat aacagtcagc cttaccagta aaaaagaaaa
32401 cctattaaaa aaacaccact cgacacggca ccagctcaat cagtcacagt gtaaaaaagg
32461 gccaagtgca gagcgagtat atataggact aaaaaatgac gtaacggtta aagtccacaa
32521 aaaacaccca gaaaaccgca cgcgaaccta cgcccagaaa cgaaagccaa aaaacccaca
32581 acttcctcaa atcgtcactt ccgttttccc acgttacgtc acttcccatt ttaagaaaac
32641 tacaattccc aacacataca agttactccg ccctaaaacc tacgtcaccc gccccgttcc
32701 cacgccccgc gccacgtcac aaactccacc ccctcattat catattggct tcaatccaaa
32761 ataaggtata ttattgatga tgttaattaa tttaaatccg catgcgatat cgagctctcc
32821 cgggaattcg gatctgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct
32881 tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac
32941 catcagggac agcttcacgg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
33001 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
33061 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
33121 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
33181 ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
33241 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
33301 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
33361 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
33421 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
33481 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
33541 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
33601 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
33661 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatca atctaaagta
33721 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
33781 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
33841 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
33901 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
33961 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
34021 gttcgccagt taatagtttg cgcaacgttg ttgccattgn tgcaggcatc gtggtgtcac
34081 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
34141 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
34201 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
34261 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
34321 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
34381 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
34441 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
34501 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
34561 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
34621 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
34681 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
34741 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
34801 ttcgtcttca aggatccgaa ttcccgggag agctcgatat cgcatgcgga tttaaattaa
34861 ttaa
//

质粒菌株产品操作说明书
一、扩增流程
         收到产品后，请先根据产品管壁标签来判断产品形式，并在扩增前准确查找该质粒菌株的抗性、感受态和培养温度。
         1、质粒干粉（常温运输，存于-20度，90天保质期，请务必转化挑单克隆培养，不要直接使用和测序）
              ①收到质粒干粉后请先5000rpm离心1min，再加入20μl ddH2O去离子水溶解质粒；
              ②取1支100μl 感受态于冰上解冻10min，加入2μl质粒，再冰浴30min后，42℃热激60s，不要搅动，再冰浴2min；（从第二步开始均要在超净工作台中无菌操作）
              ③加入900μl无抗的LB液体培养基，180rpm震荡37℃培养45min；
              ④6000rpm离心5min，仅留100μl上清液重悬细菌沉淀，并涂布至目标质粒抗性的LB平板上；（可使用本平台的平板涂布专用玻璃珠进行涂布，可以比传统涂布方法获得更多转化子）
              ⑤将平板正向培养1h，再倒置37℃培养14h。如果要求是30度则培养20h；
（菌落过多则将质粒稀释后再转化。没有菌落则加入10μl质粒转化。另不要直接转表达感受态，要先转克隆感受态，重提质粒后再导入表达感受态）
              ⑥挑取单菌落至LB液体培养基中，加入对应抗生素，220rpm震荡培养14h，根据实验需要和质粒提取试剂盒说明书提取质粒。
        2、甘油菌种（冰袋运输，存于-80℃，保质期90天，请务必划线挑单克隆培养）
              四区划线后挑单菌落培养，酵母菌需要先液体复苏再四区划线，再挑单菌落液体培养。
        3、穿刺菌种（冰袋运输，存于4℃，保质期7天）
              穿刺接种，液体培养后四区划线，再挑单菌落液体培养。
        4、菌落平板（冰袋运输，存于4℃，保质期7天）
              直接挑取单菌落至液体培养基中。
        5、液体质粒（冰袋运输，存于-20℃，保质期90天）
              单独提取的液体质粒收到后可直接使用。
        6、滤纸质粒（常温运输，存于-20度，90天保质期，请务必转化挑单克隆培养，不要直接使用和测序）
              收到货后将滤纸画圈部分剪下放入EP管中，加100ul无菌水将滤纸浸湿并浸泡5min，吸取5ul质粒转化，离心全涂。

二、转化图片