低氧诱导因子2α(HIF2a)重组蛋白(多属种)

保存条件 ：负80度

保质期 ：12个月

供应商 ：钦诚生物

规格 ：10UG/50UG

[ PROPERTIES ]
Source: Prokaryotic expression
Host: E.coli
Residues: Cys339~Gln541
Tags: N-terminal His Tag
Subcellular Location: Secreted
Purity: > 95%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Original Concentration: 50µg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 4.4
Predicted Molecular Mass: 23.6kDa
Accurate Molecular Mass: 27kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved
to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in ddH2O to a concentration of 0-0.1 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
 Store at 2-8ºC for one month.
 Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined
by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no
obvious degradation and precipitation were observed. The loss rate is less than 5% within the
expiration date under appropriate storage condition.