Mouse Immunoglobulin Isotyping Kit

Mouse Immunoglobulin Isotyping Kit

价格: ¥6160

品牌:BD

货号:550026

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库存 :大量

英文名 :Mouse Immunoglobulin Isotyping Kit

规格 :100T

小鼠IG分型试剂盒 Catalog Number
550026
Brand
BD™ Cytometric Bead Array (CBA)
Name
Mouse Immunoglobulin Isotyping Kit
Contents
Reagents for capture of mouse IgG1, IgG2a, IgG2b, IgG3, IgA, IgM, and IgE; anti-kappa and anti-lambda detection reagents; standards; buffers; cytometer setup reagents
Size
100 Tests
Regulatory Status
RUO

Description

The BD™ CBA system combines a conventional immunoassay with the sensitivity of fluorescence via flow cytometry. Each bead in a BD CBA provides a capture surface for a specific protein and is analogous to an individually coated well in an ELISA plate. The broad dynamic range of fluorescence detection, combined with multiple analyte detection, allows for small sample volume, fewer sample dilutions, and substantially less time overall to establish the value of an unknown versus a conventional ELISA approach. The Mouse Immunoglobulin Isotyping CBA Kit can be used to rapidly identify the heavy and light chain isotypes of a mouse monoclonal antibody in a single sample.

Components:

Mouse Ig Capture Bead Array - 1 vial, 5.0 mL
Anti-Mouse Kappa PE Detector Antibody (10x) - 1 vial, 0.5 mL
Anti-Mouse Lambda FITC Detector Antibody (10x) - 1 vial, 0.5 mL
Mouse Ig Standards 1 (10x) - 1 vial, 0.5 mL
Mouse Ig Standards 2 (10x) - 1 vial, 0.5 mL
Cytometer Setup Beads - 1 vial, 1.5 mL
PE Positive Control Detector - 1 vial, 0.5 mL
FITC Positive Control Detector - 1 vial, 0.5 mL
PBS Detergent Solution (10×) - 1 vial, 50 mL
文献:
  1. Chen, R., L. Lowe, J.D. Wilson, E. Crowther, K. Tzeggai, J.E. Bishop, and R. Varro. 1999. Simultaneous quantification of six human cytokines in a single sample using microparticle-based flow cytometric technology. Clin. Chem. 9: 1693-1694.

  2. Cook, E.B., J.L. Stahl, L. Lowe, R. Chen, E. Morgan, J. Wilson, R. Varro, A. Chan, F.M. Graziano, and N.P. Barney. 2001. Simultaneous measurement of six cytokines in a single sample of human tears using microparticle-based flow cytometry: allergics vs. non-allergics. J. Immunol. Methods 254: 109-118.

  3. Dotti, G., B. Salvodo, S. Takahashi, T. Goltsova, M. Brown, D. Rill, C. Rooney, and M. Brenner. 2001. Adenovector-induced expression of human-CD40-ligand (hCD40L) by multiple myeloma cells: A model for immunotherapy. Exp. Hematol. 29: 952-961.

  4. Stall, A., Q. Sun, R. Varro, L. Lowe, E. Crowther, B. Abrams, J. Bishop, and K. Davis. 1998. A single tube flow cytometric multibead assay for isotyping mouse monoclonal antibodies. Abstract LB77. Experimental Biology Meeting 1998 (late-breaking abstracts).




肿瘤研究,细胞因子,趋化因子,炎症因子,过敏毒素研究
BD生物科学一直致力于为科研工作者提供完整的流式解决方案。BDTMCBA(Cytometric Bead Array)技术是基于包被抗体的微球并利用流式细胞仪同时对多种蛋白质进行定量分析的创新性技术。每种微球具有特定的荧光强度,可以在一个试管中混合同 时进行检测。与传统的ELISA和Western Blot技术相比,该种方法需要的样本量小,检测速度更快。BDTMCBA试剂盒包括:固定待测指标预先组合的CBA kits、可配置检测方法、研究者可自由组合检测指标的CBA Flex kits。BDTMCBA技术可以应用于药物研发、免疫疾病(细胞因子)、肿瘤生物学等众多领域研究,它已经被全世界科研工作者广泛使用,相应发表的文献众多。

BD CBA技术的检测对象是分泌到液相样本中的可溶性蛋白,如细胞因子、趋化因子、炎症因子等等;目前固定好的试剂盒有Th1/Th2 CBA kit,Th1/Th2/Th17CBA kit,趋化因子CBA kit,炎症因子CBA kit及过敏毒素CBA kit等。自由组合的试剂可检测的种类 人的有61种,小鼠的27种,大鼠的6种。具体的因子如下:
小鼠 大鼠
IFN-γ,INF-α
IL-1 α,IL-1β, IL-2,IL-3, IL-4 , IL-5, IL-6,
IL-7, IL-8, IL-9, IL-10, IL-11, IL-12,
IL-12p70, IL-13, IL-17A, IL-21,
IL-23p40
TNF,TNFRⅠ,TNFRⅡ
IP-10
I-TAC
MIG
MCP-1
MIP-1 α, MIP-1 β
Eotaxin
RANTES
CD54(ICAM-1)
CD62E,CD62P,
CD62L
CD106(VCAM-1)
sCD40
sCD154(CD40 Ligand)
sCD121a,sCD121b
Soluble CD14
Basic
FGF
VEGF
Angiogenin
G-CSF,GM-CSF
LIF
LT-α
OSM
Granzyme
A, Granzyme B
Fas Ligand(FasL)
Fractalkine
IgA, IgG1, IgG2, IgG3, IgG4, IgM,
Total IgG,
IFN- γ
IL-1 α ,IL-1β, IL-2,IL-3, IL-4 , IL-5, IL-6, IL-9, IL-10, IL-12, IL-12p70, IL-13, IL-17A, IL-21, IL-23p40,
TNF
MIG
MCP-1
MIP-1 α, MIP-1 β
RANTES
KC
CD62E(E-Selectin), CD62L(L-Selectin)
G-CSF,GM-CSF
IFN- γ

IL-1 α, IL-4 , IL-6, IL-10

TNF

上海研卉生物科技有限公司

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入驻年限:10年

联系人:李先生

地址:上海

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