ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173]价格

免疫原：Carrier-protein conjugated synthetic peptide surrounding phospho Thr202/Tyr204 of human ERK1. The exact sequence is proprietary.

亚型：IgG

形态：Liquid

保存条件：Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

克隆性：Monoclonal

标记物：Unconjugated

适应物种：Human, Mouse, Rat, Drosophila

保质期：12 months from the shipping date of the product.

抗原来源：Human

目录编号：GTX635617

级别：Primary Antibodies

库存：Available

供应商：GeneTex

宿主：Rabbit

应用范围：WB, ICC/IF, IHC-P

浓度：1 mg/ml (Please refer to the vial label for the specific concentration.)

靶点：ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187)

抗体英文名：ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173]

抗体名：ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) 抗体 [HL173]

规格：100 μl/25 μl

Wild-type (WT) and ERK knockout (KO) 293T cell extracts (15 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Untreated (–) and treated (+) PC-12 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] detects ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) stained by ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Untreated (–) and treated (+) Rat2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) Neuro2A whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] detects ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) protein at endoplasmic reticulum and cytoplasm by immunofluorescent analysis.
Sample: NIH-3T3 cells were fixed in 4% PFA at RT for 15 min.
Green: ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) stained by ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Untreated (–) and treated (+) A431 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody [HL173] (GTX635617) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

该产品被引用文献

Application Reference
Chung WP et al., Sci Rep 2022 (PMID:34997132)
Watanabe M et al., Molecules 2021 (PMID:35011407)
Tsai HJ et al., Sci Rep 2021 (PMID:33436885)
Liu JF et al., J Cell Physiol 2020 (PMID:32808296)