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免疫原 :Carrier-protein conjugated synthetic peptide surrounding mutant G12D of human K-Ras. The exact sequence is proprietary.
亚型 :IgG
形态 :Liquid
保存条件 :Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性 :Monoclonal
标记物 :Unconjugated
适应物种 :Human, Mouse
保质期 :12 months from the shipping date of the product.
抗原来源 :Human
目录编号 :GTX635362
级别 :Primary Antibodies
库存 :Available
供应商 :GeneTex
宿主 :Rabbit
应用范围 :WB, ICC/IF, IHC-P
浓度 :1.5 mg/ml (Please refer to the vial label for the specific concentration.)
靶点 :RAS (G12D Mutant)
抗体英文名 :RAS (G12D Mutant) antibody [HL10]
抗体名 :RAS (G12D Mutant) 抗体 [HL10]
规格 :100 μl/25 μl
RAS (G12D Mutant) antibody [HL10] detects RAS (G12D Mutant) protein by immunohistochemical analysis.Sample: Paraffin-embedded human wild-type (left) and KRAS G12D mutant (right) pancreatic tumor.RAS (G12D Mutant) stained by RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:50.
Various whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:1000.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
RAS (G12D Mutant) antibody [HL10] detects RAS (G12D Mutant) protein at by immunohistochemical analysis.Sample: Paraffin-embedded KPC mouse pancreatic tumor cells implanted in the pancreas of B6 mice (left) and normal mouse pancreas (right).RAS (G12D Mutant) stained by RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:75.Antigen Retrieval: Citrate buffer, pH 6.0, 40 min
RAS (G12D Mutant) antibody [HL10] detects RAS (G12D Mutant) protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% PFA at RT for 15 min.Green: RAS (G12D Mutant) stained by RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
Non-transfected (–) and transfected (+) Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS (G12D Mutant) antibody [HL10] (GTX635362) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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入驻年限:12年