Recombinant Human CD27/TNFRSF7

英文名 ：Recombinant Human CD27/TNFRSF7

规格 ：10ug/50ug/500ug/1mg

Recombinant Human CD27/TNFRSF7  (CX60)

 

产品说明(Description)

Recombinant Human CD27 is produced by our Mammalian expression system and the target gene encoding Thr21-Ile192 is expressed with a Fc, 6His tag at the C-terminus. 

Accession #: P26842

Known as: CD27 antigen;CD27L receptor; T-cell activation antigen CD27; Tumor necrosis factor receptor superfamily member 7; T14 and TNFRSF7.

 

制剂（Formulation）

Lyophilized from a 0.2 μm filtered solution of 20mM Tris,150mM NaCl, pH 8.0.

 

质量控制（Quality Control） 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

Elisa:            Immobilized Human TNFSF7/CD70 (Cat:CI06) at 10μg/ml (100 μl/well) can bind biotinylated Human CD27/TNFRSF7 (Cat:CD60), The ED50 of Human CD27/TNFRSF7(Cat:CD60) is  1.72 ug/ml .

复溶（Reconstitution）

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存（Storage）

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景（Background）

CD27 antigen is also known as CD27L receptor, T-cell activation antigen CD27, Tumor necrosis factor receptor superfamily member 7, T14 and TNFRSF7. In humans, it is encoded by the CD27 gene. CD27 is a single-pass type I membrane protein with 3 TNFR-Cys repeats. It is a member of the TNF-receptor superfamily and is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. It plays a role in survival of activated T-cells and apoptosis through association with SIVA1.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY