Biotinylated Human Siglec-15 (C-Fc-Avi)

英文名 ：Biotinylated Human Siglec-15 (C-Fc-Avi)

规格 ：20μg/100μg

Biotinylated Human Siglec-15 (C-Fc-Avi)(CY14)

产品说明(Description)

Biotinylated Recombinant Human Sialic Acid-binding Ig-like Lectin 15 is produced by our Mammalian expression system and the target gene encoding Phe20-Thr263 is expressed with a Fc, Avi tag at the C-terminus.

Accession #: Q6ZMC9

Known as: Sialic acid-binding Ig-like lectin 15; Siglec-15; CD33 antigen-like 3; CD33L3

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS, 150mM NaCl, 0.3% Chaps, 5% Trehalose, pH 7.4.

 

质量控制(Quality Control)

Purity:           Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin:    Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.

Biotin:Protein Ratio: The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

 

Bioactivity-Elisa:		Bioactivity-BLI:
Immobilized Human Siglec-15-Fc-Avi (Cat#CY14) at 2μg/ml (100 μl/well) can bind Anti-Human Siglec15 mAb. The ED50 of Anti-Human Siglec15 mAb is 13.7 ng/ml.		Loaded Human Siglec-15-Fc(at#CY14) on AHC Biosensor, can bind Anti-Human Siglec15 mAb-mFc(Cat#NC007) with an affinity constant of 0.13 nM as determined in BLI assay.

 

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

 

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

 

背景(Background)

Human Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO−-terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains.  Human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY.