Biotinylated Human PD-1 (C-6His-Avi)

英文名 ：Biotinylated Human PD-1 (C-6His-Avi)

规格 ：20μg/100μg

Biotinylated Human PD-1 (C-6His-Avi) (CY18)

产品说明(Description)

Biotinylated Recombinant Human Programmed Cell Death Protein 1 is produced by our Mammalian expression system and the target gene encoding Pro21-Gln167 is expressed with a 6His, Avi tag at the C-terminus.

Accession #: Q15116

Known as:Programmed cell death protein 1; hPD-1; PDCD1; CD279 

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

 

质量控制(Quality Control) 

Purity:        Greater than 95% as determined by reducing SDS-PAGE.   

Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

Bioactivity-BLI:		Bioactivity-ELISA:
Loaded Anti-Human PD-1 mAb-Fc(Cat#GMP-A085) on Protein A Biosensor, can bind Biotinylated Human PD-1-His-Avi(Cat#CY18) with an affinity constant of 1.54 nM as determined in BLI assay.		Immobilized Human PD-1-His-Avi(Cat#CY18) at 2μg/ml (100 μl/well) can bind Anti-Human PD-1 mAb(Cat#GMP-A085). The ED50 of Human Anti-Human PD-1 mAb(Cat#GMP-A085) is 8.83 ng/ml.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.

Reconstituted protein solution can be stored at 4-7°C for 2-7 days.

Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

Programmed cell death protein 1(PDCD1) is a single-pass type I membrane protein and contains 1 Ig-like V-type domain. PD-1 is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PDCD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PDCD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY.