Reagents

Agarose, Ultrapure

Ampli Taq polymerase 5 U/µl

Buffer, 10X and 25 mM MgCl 2

Loading buffer, 5X

Ethidium Bromide

Primer “UN1”

10X TAE buffer

Template DNA

Use 400 – 600 ng for each reaction

100 mM dNTP nucleotides

x – dUTP

Materials and Equipment

PCR Thermocycler

MJ – Research, Inc., Model PTC - 100

Gel system and power source

PCR tubes

PGC Scientifics, Cat. 502-075