Base:

Bacto Heart Infusion Broth	1.2%
Bacto Yeast Extract	0.5%
Bacto Peptone	0.5%
NaCl	0.025%
Na-DNA	0.002%

pH is adjusted to 8 and autoclaved.

May be stored at room temperature for 4 weeks

ADD (to final conc.)

K2HPO4	0.17%
Glucose	1.0%
Thallium Acetate	0.025%
Penicillin	500u/ml
Horse Serum	10%
Na-DNA	0.0025%

pH to 7.8

4% culture is added and grown statically at 37°C for 18-20 hours to A260