发表在
Nucleic Acids Research, doi:10.1093/nar/gkn294
Advance Access published online on May 16, 2008

摘要：Bisulfite sequencing, a standard method for DNA methylation profile analysis, is widely used in basic and clinical studies. This method is limited, however, by the time-consuming data analysis processes required to obtain accurate DNA methylation profiles from the raw sequence output of the DNA sequencer, and by the fact that quality checking of the results can be influenced by a researcher's bias. We have developed an interactive and easy-to-use web-based tool, QUMA (quantification tool for methylation analysis), for the bisulfite sequencing analysis of CpG methylation. QUMA includes most of the data-processing functions necessary for the analysis of bisulfite sequences. It also provides a platform for consistent quality control of the analysis.

文章还没有看完，我做的是BSP+直接测序法，最近一直在考虑数据的分析方法，对于甲基化+测序法的数据统计一直都有很大的疑问，不知道怎样统计才能得到客观并且逻辑严密的结果，在此抛砖引玉，欢迎广大做甲基化的战友讨论！