请大侠帮忙看一下用下列两引物进行PCR扩增有没有可行性
上游引物：5'-GCCAGACTGGGAAGAAATCTG-3’
下游引物：5'-TGTGTTGGAAAATCCAAGTCA-3’
基因序列：
　　1 aggcagtgga gccccggcgg cggcggcggc ggcgcgcggg ggcgacgcgc gggaacaacg
       61 cgagtcggcg cgcgggacga agaataatca tgggccagac tgggaagaaa tctgagaagg
      121 gaccagtttg ttggcggaag cgtgtaaaat cagagtacat gcgactgaga cagctcaaga
      181 ggttcagacg agctgatgaa gtaaagagta tgtttagttc caatcgtcag aaaattttgg
      241 aaagaacgga aatcttaaac caagaatgga aacagcgaag gatacagcct gtgcacatcc
      301 tgacttctgt gagctcattg cgcgggacta gggagtgttc ggtgaccagt gacttggatt
      361 ttccaacaca agtcatccca ttaaagactc tgaatgcagt tgcttcagta cccataatgt
      421 attcttggtc tcccctacag cagaatttta tggtggaaga tgaaactgtt ttacataaca
      481 ttccttatat gggagatgaa gttttagatc aggatggtac tttcattgaa gaactaataa
      541 aaaattatga tgggaaagta cacggggata gagaatgtgg gtttataaat gatgaaattt
      601 ttgtggagtt ggtgaatgcc cttggtcaat ataatgatga tgacgatgat gatgatggag
      661 acgatcctga agaaagagaa gaaaagcaga aagatctgga ggatcaccga gatgataaag
      721 aaagccgccc acctcggaaa tttccttctg ataaaatttt tgaagccatt tcctcaatgt
      781 ttccagataa gggcacagca gaagaactaa aggaaaaata taaagaactc accgaacagc
      841 agctcccagg cgcacttcct cctgaatgta cccccaacat agatggacca aatgctaaat
      901 ctgttcagag agagcaaagc ttacactcct ttcatacgct tttctgtagg cgatgtttta
      961 aatatgactg cttcctacat ccttttcatg caacacccaa cacttataag cggaagaaca
     1021 cagaaacagc tctagacaac aaaccttgtg gaccacagtg ttaccagcat ttggagggag
     1081 caaaggagtt tgctgctgct ctcaccgctg agcggataaa gaccccacca aaacgtccag
     1141 gaggccgcag aagaggacgg cttcccaata acagtagcag gcccagcacc cccaccatta
     1201 atgtgctgga atcaaaggat acagacagtg atagggaagc agggactgaa acggggggag
     1261 agaacaatga taaagaagaa gaagagaaga aagatgaaac ttcgagctcc tctgaagcaa
     1321 attctcggtg tcaaacacca ataaagatga agccaaatat tgaacctcct gagaatgtgg
     1381 agtggagtgg tgctgaagcc tcaatgttta gagtcctcat tggcacttac tatgacaatt
     1441 tctgtgccat tgctaggtta attgggacca aaacatgtag acaggtgtat gagtttagag
     1501 tcaaagaatc tagcatcata gctccagctc ccgctgagga tgtggatact cctccaagga
     1561 aaaagaagag gaaacaccgg ttgtgggctg cacactgcag aaagatacag ctgaaaaagg
     1621 acggctcctc taaccatgtt tacaactatc aaccctgtga tcatccacgg cagccttgtg
     1681 acagttcgtg cccttgtgtg atagcacaaa atttttgtga aaagttttgt caatgtagtt
     1741 cagagtgtca aaaccgcttt ccgggatgcc gctgcaaagc acagtgcaac accaagcagt
     1801 gcccgtgcta cctggctgtc cgagagtgtg accctgacct ctgtcttact tgtggagccg
     1861 ctgaccattg ggacagtaaa aatgtgtcct gcaagaactg cagtattcag cggggctcca
     1921 aaaagcatct attgctggca ccatctgacg tggcaggctg ggggattttt atcaaagatc
     1981 ctgtgcagaa aaatgaattc atctcagaat actgtggaga gattatttct caagatgaag
     2041 ctgacagaag agggaaagtg tatgataaat acatgtgcag ctttctgttc aacttgaaca
     2101 atgattttgt ggtggatgca acccgcaagg gtaacaaaat tcgttttgca aatcattcgg
     2161 taaatccaaa ctgctatgca aaagttatga tggttaacgg tgatcacagg ataggtattt
     2221 ttgccaagag agccatccag actggcgaag agctgttttt tgattacaga tacagccagg
     2281 ctgatgccct gaagtatgtc ggcatcgaaa gagaaatgga aatcccttga catctgctac
     2341 ctcctccccc tcctctgaaa cagctgcctt agcttcagga acctcgagta ctgtgggcaa
     2401 tttagaaaaa gaacatgcag tttgaaattc tgaatttgca aagtactgta agaataattt
     2461 atagtaatga gtttaaaaat caacttttta ttgccttctc accagctgca aagtgttttg
     2521 taccagtgaa tttttgcaat aatgcagtat ggtacatttt tcaactttga ataaagaata
     2581 cttgaacttg tcaaaaaaaa