Transgenic Mouse and Gene Targeting Facility

Tg 008 Southern Blotting

Materials and Equipment

Hybaid hybridization oven (ISC BioExpress,H-9250)

Vacuum oven (VWR,52201-218)

Ludlum Geiger Counter

Radiation Shields and lock box (from various vendors)

Mini-centrifuge (VWR,20668-212)

Thermolyne Dri-Bath,Type 17600 (VWR,35756-006)

Platform Shaker,New Brunswick Scientific (VWR,40000-300)

Electrophoresis Separation Systems (Owl)

QIAquick Nucleotide Removal Kit (Qiagen,28304)

Prime-It II Random Primer Labeling Kit (Stratagene,300385)

[alpha-³²P]dCTP (ICN)

Salmon Sperm DNA (Stratagene,201190)

SDS solution,20% w/v (Bio-Rad,161-0418)

Zeta-Probe Genomic Tested Blotting Membrane (nylon)(Bio-Rad,162-0196)

Gel Blot Paper GB002 (Schleicher